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Information Journal Paper

Title

CLONING AND EXPRESSION OF BRUCELLA CYCLIC β-1, 2 GLUCAN TRANSPORTER GENE (CGT)

Pages

  3-7

Abstract

 Background: Brucellosis is an important cosmopolitan infection disease caused by organisms belonging to the genus BRUCELLA. The cgt gene (cyclic b-1, 2 glucan transporter gene) is a virulent factor in BRUCELLA genus. The present study was conducted with the aim of cloning and expression of BRUCELLA cgt gene. Materials and methods: BRUCELLA melitensis cgt gene was amplified from extracted chromosomal DNA by PCR, then PCR product was cloned into pTZ57R and subcloned into pGEMEX-1 expression vector, then expressed in JM109 E.coli strain. RECOMBINANT PROTEIN was confirmed by western blot analysis using patient's serum. Results: The PCR product was cloned in pTZ57R plasmid via T/A cloning method. Recombinant plasmid was digested by BamHI and SacI restriction enzymes, the released band was purified and subcloned into pGEMEX-1 expression vector. Then, sample cells were lysed using lyses buffer and sonicated, then electrophoresed on SDS-PAGE. Protein bands were transferred on nitrocellulose membrane and reacted by patient's serum and detected by HRP conjugated anti human antibody.Conclusion: We cloned and expressed BRUCELLA abortus cyclic b-1, 2-glucan transporter gene (cgt) which is an important agent in brucellosis. Using cgt gene mutant may be an effective way for inhibiting or decreasing the pathogenicity of bacteria.

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    APA: Copy

    BANDEHPOUR, M., ABDALI, N., SADEGHI, FARZANEH, PARIVAR, KAZEM, SHARIFNIA, Z., RIAHI, H., HAGHIGHI, ALI, & KAZEMI, BAHRAM. (2009). CLONING AND EXPRESSION OF BRUCELLA CYCLIC β-1, 2 GLUCAN TRANSPORTER GENE (CGT). IRANIAN JOURNAL OF CLINICAL INFECTIOUS DISEASES, 4(1), 3-7. SID. https://sid.ir/paper/313499/en

    Vancouver: Copy

    BANDEHPOUR M., ABDALI N., SADEGHI FARZANEH, PARIVAR KAZEM, SHARIFNIA Z., RIAHI H., HAGHIGHI ALI, KAZEMI BAHRAM. CLONING AND EXPRESSION OF BRUCELLA CYCLIC β-1, 2 GLUCAN TRANSPORTER GENE (CGT). IRANIAN JOURNAL OF CLINICAL INFECTIOUS DISEASES[Internet]. 2009;4(1):3-7. Available from: https://sid.ir/paper/313499/en

    IEEE: Copy

    M. BANDEHPOUR, N. ABDALI, FARZANEH SADEGHI, KAZEM PARIVAR, Z. SHARIFNIA, H. RIAHI, ALI HAGHIGHI, and BAHRAM KAZEMI, “CLONING AND EXPRESSION OF BRUCELLA CYCLIC β-1, 2 GLUCAN TRANSPORTER GENE (CGT),” IRANIAN JOURNAL OF CLINICAL INFECTIOUS DISEASES, vol. 4, no. 1, pp. 3–7, 2009, [Online]. Available: https://sid.ir/paper/313499/en

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