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Information Journal Paper

Title

DEVELOPMENT OF AN IMMUNOAFFINITY METHOD FOR PURIFICATION OF STREPTOKINASE

Pages

  142-147

Abstract

 Background: STREPTOKINASE is a potent activator of plasminogen to plasmin, the enzyme that can solubilize the fibrin network in blood clots. STREPTOKINASE is currently used in clinical medicine as a THROMBOLYTIC AGENT. It is naturally secreted by β-hemolytic streptococci.Methods: To reach an efficient method of PURIFICATION, an immunoaffinity CHROMATOGRAPHY method was developed that could purify the STREPTOKINASE in a single step with high yield. At the first stage, a CNBr-Ac-tivated sepharose 4B-Lysine column was made to purify the human blood plasminogen. The purified plasminogen was utilized to construct a column that could purify the STREPTOKINASE. The rabbit was immunized with the purified STREPTOKINASE and the anti-streptokinase (IgG) purified on another STREPTOKINASE substituted sepharose-4B column. The immunoaffinity column was developed by coupling the purified anti-Streptokinase (IgG) to sepharose 6MB-Protein A. The Escherichia coli (E.coli) BL21 (DE3) pLysS strain was transformed by the recombinant construct (cloned STREPTOKINASE gene in pGEX-4T-2 vector) and gene expression was induced by IPTG. The expressed protein was purified by immunoaffinity CHROMATOGRAPHY in a single step.Results: The immunoaffinity column could purify the recombinant fusion GSTSK to homogeneity. The purity of STREPTOKINASE was confirmed by SDS-PAGE as a single band of about 71 kD and its biological activity determined in a specific STREPTOKINASE assay. The yield of the PURIFICATION was about 94%. Conclusion: This method of STREPTOKINASE PURIFICATION is superior to the previous conventional methods.

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    • Cite

    APA: Copy

    KARIMI, ZOHREH, BABASHAMSI, MOHAMMAD, ASGARANI, EZAT, & SALIMI, ALI. (2012). DEVELOPMENT OF AN IMMUNOAFFINITY METHOD FOR PURIFICATION OF STREPTOKINASE. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 4(3 (14)), 142-147. SID. https://sid.ir/paper/313783/en

    Vancouver: Copy

    KARIMI ZOHREH, BABASHAMSI MOHAMMAD, ASGARANI EZAT, SALIMI ALI. DEVELOPMENT OF AN IMMUNOAFFINITY METHOD FOR PURIFICATION OF STREPTOKINASE. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2012;4(3 (14)):142-147. Available from: https://sid.ir/paper/313783/en

    IEEE: Copy

    ZOHREH KARIMI, MOHAMMAD BABASHAMSI, EZAT ASGARANI, and ALI SALIMI, “DEVELOPMENT OF AN IMMUNOAFFINITY METHOD FOR PURIFICATION OF STREPTOKINASE,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 4, no. 3 (14), pp. 142–147, 2012, [Online]. Available: https://sid.ir/paper/313783/en

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