AMPLIFICATION OF GC-RICH PUTATIVE MOUSE PEP PROMOTER USING BETAINE AND DMSO IN AMMONIUM SULFATE POLYMERASE CHAIN REACTION BUFFER (SHORT COMMUNICATION)

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SEIFI TAHERE; GHAEDI KAMRAN; SALAMIAN AHMAD; TANHAEI SOMMAYEH; SAFARI FOROUZAN; HOJATI ZOHREH; TAVASSOLI MANUCHEHR; BAHARVAND HOSSEIN; NASRESFAHANI MOHAMMAD HOSSEIN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Avicenna Journal of Medical Biotechnology Year:2012 | Volume:4 | Issue:4 (15) Page(s): 206-209

Download Full-Text

View:

381

Download:

144

Cites:

Information Journal Paper

Title

AMPLIFICATION OF GC-RICH PUTATIVE MOUSE PEP PROMOTER USING BETAINE AND DMSO IN AMMONIUM SULFATE POLYMERASE CHAIN REACTION BUFFER (SHORT COMMUNICATION)

Author(s)

Keywords

AMPLIFICATION

GC-RICH REGION

PEROXSIOMAL PROTEIN

PROMOTER

Abstract

Background: Recently, we have shown that peroxisomal protein expression was induced upon retinoic acid treatment in mouse embryonic stem cells during the process of neurogenesis. Thus, characterization of the respective PROMOTER could elucidate the molecular aspects of transcriptional regulation of this gene.Methods: Using the conventional software programs for PROMOTER prediction, a putative PROMOTER region was identified approximately 561bp upstream of the peroxisomal protein coding sequence. In order to clone this region with a GC-content of 71.01%, a cocktail of ammonium sulfate buffer supplied with two additive components, betaine and dimethyl sulfoxide, and a high concentration of MgCl2 was used.Results: The modulated polymerase chain reaction composition significantly improved the AMPLIFICATION of GC-rich DNA target sequences. Improved AMPLIFICATION of this region was due to reduction in the formation of secondary structures by the GC-RICH REGION.Conclusion: Therefore, this polymerase chain reaction composition could be generally used to facilitate the AMPLIFICATION of other GC-rich DNA sequences as verified by AMPLIFICATION of different GC rich regions.

Cites

NEW INSIGHTS INTO VEGF-A ALTERNATIVE SPLICING: KEY REGULATORY SWITCHING IN THE PATHOLOGICAL PROCESS

References

No record.

Cite

APA: Copy

SEIFI, TAHERE, GHAEDI, KAMRAN, SALAMIAN, AHMAD, TANHAEI, SOMMAYEH, SAFARI, FOROUZAN, HOJATI, ZOHREH, TAVASSOLI, MANUCHEHR, BAHARVAND, HOSSEIN, & NASRESFAHANI, MOHAMMAD HOSSEIN. (2012). AMPLIFICATION OF GC-RICH PUTATIVE MOUSE PEP PROMOTER USING BETAINE AND DMSO IN AMMONIUM SULFATE POLYMERASE CHAIN REACTION BUFFER (SHORT COMMUNICATION). AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 4(4 (15)), 206-209. SID. https://sid.ir/paper/313790/en

Vancouver: Copy

SEIFI TAHERE, GHAEDI KAMRAN, SALAMIAN AHMAD, TANHAEI SOMMAYEH, SAFARI FOROUZAN, HOJATI ZOHREH, TAVASSOLI MANUCHEHR, BAHARVAND HOSSEIN, NASRESFAHANI MOHAMMAD HOSSEIN. AMPLIFICATION OF GC-RICH PUTATIVE MOUSE PEP PROMOTER USING BETAINE AND DMSO IN AMMONIUM SULFATE POLYMERASE CHAIN REACTION BUFFER (SHORT COMMUNICATION). AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2012;4(4 (15)):206-209. Available from: https://sid.ir/paper/313790/en

IEEE: Copy

TAHERE SEIFI, KAMRAN GHAEDI, AHMAD SALAMIAN, SOMMAYEH TANHAEI, FOROUZAN SAFARI, ZOHREH HOJATI, MANUCHEHR TAVASSOLI, HOSSEIN BAHARVAND, and MOHAMMAD HOSSEIN NASRESFAHANI, “AMPLIFICATION OF GC-RICH PUTATIVE MOUSE PEP PROMOTER USING BETAINE AND DMSO IN AMMONIUM SULFATE POLYMERASE CHAIN REACTION BUFFER (SHORT COMMUNICATION),” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 4, no. 4 (15), pp. 206–209, 2012, [Online]. Available: https://sid.ir/paper/313790/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops