مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Cites:

1

Information Journal Paper

Title

CLONING AND EXPRESSION OF LEISHMANIA INFANTUM LPG3 GENE BY THE LIZARD LEISHMANIA EXPRESSION SYSTEM

Pages

  186-192

Abstract

 Background: Various prokaryotic and eukaryotic expression systems have been developed for the production of RECOMBINANT PROTEINS. In the present study, we used a new protein expression system based on the Iranian Lizard LEISHMANIA, a trypanosomatid protozoan as a host, for the expression of LPG3 gene from LEISHMANIA INFANTUM (L. infantum).Methods: The LPG3 gene was cloned in the expression cassette for integration into the small subunit of the ribosomal RNA locus of Lizard LEISHMANIA genome by electroporation. Expression of the recombinant LPG3 protein was confirmed by western blotting and immunofluorescence staining.Results: Western blotting confirmed the expression and production of rLPG3 protein. Immunofluoresence analysis also revealed the staining throughout the cytoplasm of transfected parasites, indicating that the protein has been expressed.Conclusion: These results demonstrate that LEISHMANIA cells can be suggested an expression system for the production of recombinant LPG3 (rLPG3) to further research in vaccine designing against LEISHMANIAsis.

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    • Cite

    APA: Copy

    PIRDEL, LEILA, ZAVARAN HOSSEINI, AHMAD, KAZEMI, BAHRAM, RASOULI, MANOOCHEHR, BANDEHPOUR, MOJGAN, & SOUDI, SARA. (2012). CLONING AND EXPRESSION OF LEISHMANIA INFANTUM LPG3 GENE BY THE LIZARD LEISHMANIA EXPRESSION SYSTEM. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 4(4 (15)), 186-192. SID. https://sid.ir/paper/313796/en

    Vancouver: Copy

    PIRDEL LEILA, ZAVARAN HOSSEINI AHMAD, KAZEMI BAHRAM, RASOULI MANOOCHEHR, BANDEHPOUR MOJGAN, SOUDI SARA. CLONING AND EXPRESSION OF LEISHMANIA INFANTUM LPG3 GENE BY THE LIZARD LEISHMANIA EXPRESSION SYSTEM. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2012;4(4 (15)):186-192. Available from: https://sid.ir/paper/313796/en

    IEEE: Copy

    LEILA PIRDEL, AHMAD ZAVARAN HOSSEINI, BAHRAM KAZEMI, MANOOCHEHR RASOULI, MOJGAN BANDEHPOUR, and SARA SOUDI, “CLONING AND EXPRESSION OF LEISHMANIA INFANTUM LPG3 GENE BY THE LIZARD LEISHMANIA EXPRESSION SYSTEM,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 4, no. 4 (15), pp. 186–192, 2012, [Online]. Available: https://sid.ir/paper/313796/en

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