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Information Journal Paper

Title

EXPRESSION AND PURIFICATION OF RECOMBINANT ROP1 OF TOXOPLASMA GONDII IN BACTERIA

Pages

  227-233

Abstract

 cause serious health problems in congenitally infected newborns and immunecompromised individuals. Research is undergoing both to improve Toxoplasma serological tests, which play the main role in laboratory diagnosis of the infection, and develop an effective vaccine to prevent the infection. Some studies showed usefulness of rhoptry protein 1 (ROP1) antigen of TOXOPLASMA GONDII (T. gondii) in serodiagnosis of the infection and induction of protective immunity. The purpose of this study was to produce recombinant ROP1 and evaluate its antigenicity against human infected sera.Methods: DNA encoding ROP1, amino acids 171 to 574, was obtained from T. gondii RH strain by polymerase chain reaction amplification and cloned in prokaryotic expression plasmid pET-15b. rROP1 was expressed in Escherichia coli (E. coli)and purified in a single step by immobilized metal ion affinity chromatography.Results: DNA sequencing showed 99% similarity between the cloned sequence and the corresponding sequence in Gene bank. Results indicated the proper antigenicity of rROP1. Sera from Toxoplasma infected individuals specifically recognized rROP1 in Western blotting.Conclusion: rROP1 is antigenic toward human infected sera and can be used in studies for development of both a Toxoplasma serological test and a protective vaccine.

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    Cite

    APA: Copy

    MOHABATI, REYHANEH, BABAIE, JALAL, AMIRI, SAMIRA, TALEBZADEH, MOHAMMAD, FARD ESFAHANI, PEZHMAN, DARBOUY, MOJTABA, & GOLKAR, MAJID. (2013). EXPRESSION AND PURIFICATION OF RECOMBINANT ROP1 OF TOXOPLASMA GONDII IN BACTERIA. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 5(4 (18)), 227-233. SID. https://sid.ir/paper/313818/en

    Vancouver: Copy

    MOHABATI REYHANEH, BABAIE JALAL, AMIRI SAMIRA, TALEBZADEH MOHAMMAD, FARD ESFAHANI PEZHMAN, DARBOUY MOJTABA, GOLKAR MAJID. EXPRESSION AND PURIFICATION OF RECOMBINANT ROP1 OF TOXOPLASMA GONDII IN BACTERIA. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2013;5(4 (18)):227-233. Available from: https://sid.ir/paper/313818/en

    IEEE: Copy

    REYHANEH MOHABATI, JALAL BABAIE, SAMIRA AMIRI, MOHAMMAD TALEBZADEH, PEZHMAN FARD ESFAHANI, MOJTABA DARBOUY, and MAJID GOLKAR, “EXPRESSION AND PURIFICATION OF RECOMBINANT ROP1 OF TOXOPLASMA GONDII IN BACTERIA,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 5, no. 4 (18), pp. 227–233, 2013, [Online]. Available: https://sid.ir/paper/313818/en

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