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Information Journal Paper

Title

EVALUATION OF THE EFFECT OF MIR-26B UP-REGULATION ON HBF EXPRESSION IN ERYTHROLEUKEMIC K-562 CELL LINE

Pages

  53-56

Keywords

Abstract

 Background: The major hemoglobin in the fetus is hemoglobin F (HbF) (a2 g2), whereas in adult humans, hemoglobin A (a2b2) is predominately expressed. Several studies have indicated that expression of the HbF subunit g-globin might be regulated post-transcriptionally. This could be done by small non-coding RNAs called MICRORNAS which target mRNAs in a sequencespecific manner and lead to translational repression or mRNA decay. The aim of this study is to evaluate the effect of MIR-26B up-regulation on g-globin gene expression in K-562 cell line.Methods: These cells were grown in RPMI 1640 and pre MIR-26B and were transfected within K-562 cell line using lentiviral vector. After RNA extraction and cDNA synthesis in selected days, miRNA up-regulation was confirmed by miRNA real time PCR and then gand bchain and GATA-1 expression were investigated by RT and QRT-PCR.Results: The viability of cells before transfection was 90%. Three and 7 days after transfection, through the use of relative Q-PCR, the g chain expression increased 3.7, 6.8 and 3.8 folds and GATA-1 expression increased 2.1, 6.0 and 8.0 in comparison with untransfected cells.Conclusion: The data suggest that MIR-26B can be involved in the increase of g-globin gene expression in K-562 cell line. We suggest that MIR-26B may be a significant therapeutic target for increasing HbF levels in patients with sickle cell disease and b-thalassemia.

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    Cite

    APA: Copy

    ALIJANI, SADEGH, ALIZADEH, SHABAN, KAZEMI, AHMAD, KASHANI KHATIB, ZAHRA, SOLEIMANI, MASOUD, REZVANI, MOHAMAD REZA, MINAYI, NEDA, KARAMI, FARSHID, & TAYEBI, BEHNOOSH. (2014). EVALUATION OF THE EFFECT OF MIR-26B UP-REGULATION ON HBF EXPRESSION IN ERYTHROLEUKEMIC K-562 CELL LINE. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 6(1), 53-56. SID. https://sid.ir/paper/313829/en

    Vancouver: Copy

    ALIJANI SADEGH, ALIZADEH SHABAN, KAZEMI AHMAD, KASHANI KHATIB ZAHRA, SOLEIMANI MASOUD, REZVANI MOHAMAD REZA, MINAYI NEDA, KARAMI FARSHID, TAYEBI BEHNOOSH. EVALUATION OF THE EFFECT OF MIR-26B UP-REGULATION ON HBF EXPRESSION IN ERYTHROLEUKEMIC K-562 CELL LINE. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2014;6(1):53-56. Available from: https://sid.ir/paper/313829/en

    IEEE: Copy

    SADEGH ALIJANI, SHABAN ALIZADEH, AHMAD KAZEMI, ZAHRA KASHANI KHATIB, MASOUD SOLEIMANI, MOHAMAD REZA REZVANI, NEDA MINAYI, FARSHID KARAMI, and BEHNOOSH TAYEBI, “EVALUATION OF THE EFFECT OF MIR-26B UP-REGULATION ON HBF EXPRESSION IN ERYTHROLEUKEMIC K-562 CELL LINE,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 6, no. 1, pp. 53–56, 2014, [Online]. Available: https://sid.ir/paper/313829/en

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