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Information Journal Paper

Title

COMPARISON BETWEEN PLATINUM-AZIDOTHYMIDINE AND AZIDOTHYMIDINE EFFECTS ON BCL-2 AND TELOMERASE GENE EXPRESSION IN RATS WITH HEPATOCELLULAR CARCINOMA

Pages

  50-56

Abstract

 Background: High expression of TELOMERASE and Bcl-2 are reported in HEPATOCELLULAR CARCINOMA. Some anticancer drugs show their effects through reduction of these factors. In this study, it was aimed to investigate the effects of a new synthetic compound, PLATINUM azidothymidine, on inhibition of TELOMERASE and Bcl-2 expression in HEPATOCELLULAR CARCINOMA compared to azidothymidine.Methods: To study the effects of Pt-AZT on HEPATOCELLULAR CARCINOMA and compare its effects with AZT in inhibition of TELOMERASE and Bcl-2 GENE EXPRESSION, pathogen-free male Wistar rats (n=100) were used. They were randomly divided to 4 groups (n=25). Group A as the control group contained 25 healthy rats; in the rest of animals, preneoplastic lesions were induced in their livers (groups B, C, and D) using Solt- Farber resistant hepatocyte protocol. Cancer development was approved by a pathology laboratory. Group B was negative control (untreated), groups C and D were treated by intraperitoneal injection (IP) of Pt-AZT (0.9 mg/kg/day) and AZT (0.3 mg/kg/day), respectively for 14 days. At the end of the protocol, all rats were sacrificed and Bcl-2 and TELOMERASE GENE EXPRESSION was determined using real -time PCR.Results: No tumor in the livers was found in group A at any point of the study, but it was present in livers of all animals in B, C and D groups. Results showed that TELOMERASE and Bcl-2 expression was significantly lower in group C compared with group B (0.473±0.231 vs.5.137±5.08, p<0.001, for TELOMERASE expression, and 0.41±0.276 vs.7.25±11.6, p<0.001, for Bcl-2 expression) and also compared with group D (0.473±0.231 vs.3.48±4.02, p<0.001, for TELOMERASE expression, and 0.41±0.276 vs.4.93±18, p<0.001, for Bcl-2 expression).Conclusion: For the first time, it was demonstrated that Pt-AZT has more inhibitory effect on TELOMERASE and Bcl-2 expression than AZT. It effectively inhibits the growth of liver tumor in rats by extending apoptosis.

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    APA: Copy

    SABOKROUH, ABDOLREZA, VAISI RAYGANI, ASAD, GOODARZI, MOHAMMAD TAGHI, KHATAMI, SHOHREH, TAGHIZADEH JAHED, MASSOUD, SHAHABADI, NAHID, LAKPOUR, NIKNAM, & SHAKIBA, YADOLLAH. (2015). COMPARISON BETWEEN PLATINUM-AZIDOTHYMIDINE AND AZIDOTHYMIDINE EFFECTS ON BCL-2 AND TELOMERASE GENE EXPRESSION IN RATS WITH HEPATOCELLULAR CARCINOMA. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 7(2), 50-56. SID. https://sid.ir/paper/313864/en

    Vancouver: Copy

    SABOKROUH ABDOLREZA, VAISI RAYGANI ASAD, GOODARZI MOHAMMAD TAGHI, KHATAMI SHOHREH, TAGHIZADEH JAHED MASSOUD, SHAHABADI NAHID, LAKPOUR NIKNAM, SHAKIBA YADOLLAH. COMPARISON BETWEEN PLATINUM-AZIDOTHYMIDINE AND AZIDOTHYMIDINE EFFECTS ON BCL-2 AND TELOMERASE GENE EXPRESSION IN RATS WITH HEPATOCELLULAR CARCINOMA. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2015;7(2):50-56. Available from: https://sid.ir/paper/313864/en

    IEEE: Copy

    ABDOLREZA SABOKROUH, ASAD VAISI RAYGANI, MOHAMMAD TAGHI GOODARZI, SHOHREH KHATAMI, MASSOUD TAGHIZADEH JAHED, NAHID SHAHABADI, NIKNAM LAKPOUR, and YADOLLAH SHAKIBA, “COMPARISON BETWEEN PLATINUM-AZIDOTHYMIDINE AND AZIDOTHYMIDINE EFFECTS ON BCL-2 AND TELOMERASE GENE EXPRESSION IN RATS WITH HEPATOCELLULAR CARCINOMA,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 7, no. 2, pp. 50–56, 2015, [Online]. Available: https://sid.ir/paper/313864/en

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