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Information Journal Paper

Title

PRELIMINARY ASSESSMENT OF VARIOUS ADDITIVES ON THE SPECIFIC REACTIVITY OF ANTI- RHBSAG MONOCLONAL ANTIBODIES

Pages

  145-150

Keywords

MONOCLONAL ANTIBODIES (MABS) 

Abstract

 Background: Antibodies have a wide application in diagnosis and treatment. In order to maintain optimal stability of various functional parts of antibodies such as antigen binding sites, several approaches have been suggested. Using additives such as poly-saccharides and polyols is one of the main methods in protecting antibodies against aggregation or degradation in the formulation. The aim of this study was to evaluate the protective effect of various additives on the specific reactivity of monoclonal antibodies (mAbs) against recombinant HBsAg (rHBsAg) EPITOPES.Methods: To estimate the protective effect of different additives on the stability of an-tibody against conformational EPITOPES (S3 antibody) and Linear linear EPITOPES (S7 and S11 antibodies) of rHBsAg, heat shock in at 37oC was performed in liquid and solid phases. Environmental factors were considered to be constant. The specific reactivity of antibodies was evaluated using an ELISA method. The data were analyzed using SPSS software by Mann-Whitney nonparametric test with the confidence interval of 95%.Results: Our results showed that 0.25 M sucrose, 0.04 M trehalose and 0.5% BSA had the most protective effect on maintaining the reactivity of mAbs (S3) against conformational EPITOPES of rHBsAg. Results obtained from S7 and S11 mAbs against linear characteristics showed minor differences. The most efficient protective additives were 0.04 M trehalose and 1 M sucrose.Conclusion: Nowadays, application of appropriate additives is more interested important for increasing the stability of antibodies. It was concluded that sucrose, trehalose and BSA have considerable effects on the specific reactivity of anti rHBsAg mAbs during long storage.

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    Cite

    APA: Copy

    YAZDANI, YAGHOUB, MOHAMMADI, SAEED, YOUSEFI, MEHDI, & SHOKRI, FAZEL. (2015). PRELIMINARY ASSESSMENT OF VARIOUS ADDITIVES ON THE SPECIFIC REACTIVITY OF ANTI- RHBSAG MONOCLONAL ANTIBODIES. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 7(4 (27)), 145-150. SID. https://sid.ir/paper/313885/en

    Vancouver: Copy

    YAZDANI YAGHOUB, MOHAMMADI SAEED, YOUSEFI MEHDI, SHOKRI FAZEL. PRELIMINARY ASSESSMENT OF VARIOUS ADDITIVES ON THE SPECIFIC REACTIVITY OF ANTI- RHBSAG MONOCLONAL ANTIBODIES. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2015;7(4 (27)):145-150. Available from: https://sid.ir/paper/313885/en

    IEEE: Copy

    YAGHOUB YAZDANI, SAEED MOHAMMADI, MEHDI YOUSEFI, and FAZEL SHOKRI, “PRELIMINARY ASSESSMENT OF VARIOUS ADDITIVES ON THE SPECIFIC REACTIVITY OF ANTI- RHBSAG MONOCLONAL ANTIBODIES,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 7, no. 4 (27), pp. 145–150, 2015, [Online]. Available: https://sid.ir/paper/313885/en

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