مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

CLONING AND EXPRESSION OF SOLUBLE RECOMBINANT HIV-1 CRF35 PROTEASE-HP THIOREDOXIN FUSION PROTEIN

Pages

  175-181

Abstract

 Background: As a drug target and an antigenic agent, HIV-1 PROTEASE (HIV-1 PR) is at the center of attention for designing anti-AIDS inhibitors and diagnostic tests. In previous studies, the production of the recombinant PROTEASE has been faced with several difficulties; therefore, the aims of this study were the easy production, purification of the soluble form of PROTEASE in E. coli and investigation of its immunoreactivity.Methods: PROTEASE coding region was isolated from the serum of an infected individual, amplified by RT-PCR and cloned into PTZ57R using TA-cloning. PROTEASE coding frame was isolated by PCR and cloned in pET102/D. TOPO expression vector and cloned PROTEASE was expressed in Escherichia coli (E. coli) BL21. Produced recombinant protein was purified by affinity Ni-NTA column and protein concentration was checked by BCA protein assay kit. Subsequently, immunoreactivity of recombinant PROTEASE (rPR) was assayed by Western blotting and ELISA.Results: Cloning of the HIV PROTEASE by TOPO cloning system in pET102/D.TOPO was confirmed with PCR and sequencing. The concentration range of purified recombinant protein was 85 to 100mg/ml. Immunogenicity of rPR was confirmed by Western blotting and ELISA.Conclusion: Soluble production of recombinant HIV-1 PROTEASE (HIV-1 rPR) was performed successfully. This recombinant protein disclosed 86% specificity and 90% sensitivity in immunoassay tests.

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    APA: Copy

    AZARNEZHAD, ASAAD, SHARIFI, ZOHREH, SEYEDABADI, RAHMATOLLAH, HOSSEINI, ARSHAD, JOHARI, BEHROOZ, & SOBHANI FARD, MAHSA. (2016). CLONING AND EXPRESSION OF SOLUBLE RECOMBINANT HIV-1 CRF35 PROTEASE-HP THIOREDOXIN FUSION PROTEIN. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 8(4), 175-181. SID. https://sid.ir/paper/313908/en

    Vancouver: Copy

    AZARNEZHAD ASAAD, SHARIFI ZOHREH, SEYEDABADI RAHMATOLLAH, HOSSEINI ARSHAD, JOHARI BEHROOZ, SOBHANI FARD MAHSA. CLONING AND EXPRESSION OF SOLUBLE RECOMBINANT HIV-1 CRF35 PROTEASE-HP THIOREDOXIN FUSION PROTEIN. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2016;8(4):175-181. Available from: https://sid.ir/paper/313908/en

    IEEE: Copy

    ASAAD AZARNEZHAD, ZOHREH SHARIFI, RAHMATOLLAH SEYEDABADI, ARSHAD HOSSEINI, BEHROOZ JOHARI, and MAHSA SOBHANI FARD, “CLONING AND EXPRESSION OF SOLUBLE RECOMBINANT HIV-1 CRF35 PROTEASE-HP THIOREDOXIN FUSION PROTEIN,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 8, no. 4, pp. 175–181, 2016, [Online]. Available: https://sid.ir/paper/313908/en

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