OPTIMIZATION OF EFFECTIVE MINERALS ON RIBOFLAVIN PRODUCTION BY BACILLUS SUBTILIS SUBSP. SUBTILIS ATCC 6051 USING STATISTICAL DESIGNS

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ORAEI MARJAN; RAZAVI SEYED HADI; KHODAIYAN FARAMARZ

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Journal: Avicenna Journal of Medical Biotechnology Year:2018 | Volume:10 | Issue:1 Page(s): 49-55

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Title

OPTIMIZATION OF EFFECTIVE MINERALS ON RIBOFLAVIN PRODUCTION BY BACILLUS SUBTILIS SUBSP. SUBTILIS ATCC 6051 USING STATISTICAL DESIGNS

Author(s)

ORAEI MARJAN | RAZAVI SEYED HADI | KHODAIYAN FARAMARZ | Issue Writer Certificate

Keywords

BACILLUS SUBTILIS ATCC 6051

MINERALS

RIBOFLAVIN

Abstract

Background: RIBOFLAVIN (vitamin B2) is an essential component of the basic metabolism, and an important nutritional and growth factor in humans, animals, plants and micro-organisms. It has been widely used in the fields of pharmaceuticals, feed and food additives. The industrial production of RIBOFLAVIN mostly relies on the microbial fermentation. Designing an appropriate fermentation medium is of crucial importance to improve the RIBOFLAVIN production.Methods: In this study, sequential methodology combining a screening test of MINERALS by Plackett-Burman (PB) and an optimization test by Central Composite Design (CCD) was applied to enhance RIBOFLAVIN production byBACILLUS SUBTILIS ATCC 6051 in shake flasks.Results: Initially, one-factor-at-a-time approach was applied to evaluate the effect of different carbon sources. The results showed that fructose was significantly most effective on biomass and RIBOFLAVIN production. After that, 13 MINERALS [CaCl2, CuCl, FeCl3, FeSO4, AlCl3, Na3MoO4, Co (NO3) 2, NaCl, KH2PO4, K2HPO4, MgSO4, ZnSO4, and MnSO4] were studied with the screening test. The results revealed that concentration of MgSO4, K2HPO4, and FeSO4 had greater influence on RIBOFLAVIN production (p< 0.05). A CCD with five factors (concentration of fructose, MgSO4, K2HPO4, FeSO4, and yeast extract) at five levels was then used to determine the maximum RIBOFLAVIN concentration. The optimal concentrations (g/l) of these variables determined by Response Surface Methodology (RSM) were fructose, 38.10; MgSO4, 0.85; K2HPO4, 2.27; FeSO4, 0.02; and yeast extract, 4.37.Conclusion: Statistical experimental design offers a practicable approach to the implementation of medium optimization. From an industrial view point, our optimum medium, besides fructose and a small amount of yeast extract, is mainly composed of common and cheap inorganic salts, which are available to the industrial RIBOFLAVIN production.

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APA: Copy

ORAEI, MARJAN, RAZAVI, SEYED HADI, & KHODAIYAN, FARAMARZ. (2018). OPTIMIZATION OF EFFECTIVE MINERALS ON RIBOFLAVIN PRODUCTION BY BACILLUS SUBTILIS SUBSP. SUBTILIS ATCC 6051 USING STATISTICAL DESIGNS. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 10(1), 49-55. SID. https://sid.ir/paper/313945/en

Vancouver: Copy

ORAEI MARJAN, RAZAVI SEYED HADI, KHODAIYAN FARAMARZ. OPTIMIZATION OF EFFECTIVE MINERALS ON RIBOFLAVIN PRODUCTION BY BACILLUS SUBTILIS SUBSP. SUBTILIS ATCC 6051 USING STATISTICAL DESIGNS. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2018;10(1):49-55. Available from: https://sid.ir/paper/313945/en

IEEE: Copy

MARJAN ORAEI, SEYED HADI RAZAVI, and FARAMARZ KHODAIYAN, “OPTIMIZATION OF EFFECTIVE MINERALS ON RIBOFLAVIN PRODUCTION BY BACILLUS SUBTILIS SUBSP. SUBTILIS ATCC 6051 USING STATISTICAL DESIGNS,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 10, no. 1, pp. 49–55, 2018, [Online]. Available: https://sid.ir/paper/313945/en

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