مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

SOLUBLE EXPRESSION OF HUMANIZED ANTI-CD20 SINGLE CHAIN ANTIBODY IN ESCHERICHIA COLI BY CYTOPLASMIC CHAPERONES CO-EXPRESSION

Pages

  141-146

Abstract

 Background: CD20 is an important cell surface receptor that is used for target therapy of B cell lymphoma and some related blood diseases due to vital function of CD20.In previous studies, a Rituximab based humanized single chain variable fragment (scFv) antibody showed good reactivity against B cell related cancer cells. But this recombinant protein produced Inclusion Bodies (IBs) inEscherichia coli (E. coli) cytoplasm.The aim of this study was to investigate the effect of coexpression with cytoplasmic chaperones on expression and solubility of humanized anti-CD20 scFv inE.coli.Methods: For this purpose, the fragment coding for anti-CD20 huscFv subcloned into the pET22b (+) and transformed into theE. coli BL21 (DE3) was evaluated. In order to inhibit the production of IBs, the effects of co-expression with cytoplasmic chaperones GroEL, DnaK, GroES, Tig, DnaJ and GrpE were investigated.Result: Coexpression with cytoplasmic chaperones led to increased soluble expression of anti-CD20 recombinant protein. Among investigated chaperones, pKJE7 chaperone plasmid containing DnaJ, GrpE, DnaK chaperone genes had significant effects with an expression yield of 325μg/ml soluble anti-CD20 scFv.Conclusion: The result of this study demonstrated remarkable effect of pKJE7 chaperone on enhancement of soluble expression of anti-CD20 huscFv antibody inE. coli.

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    APA: Copy

    YOUSEFI, MOHAMMAD REZA, FARAJNIA, SAFAR, MOKHTARZADEH, AHAD, AKBARI, BAHMAN, AHDI KHOSROSHAHI, SHIVA, MAMIPOUR, MINA, DARIUSHNEJAD, HASSAN, & Ahmadzadeh, Vahideh. (2018). SOLUBLE EXPRESSION OF HUMANIZED ANTI-CD20 SINGLE CHAIN ANTIBODY IN ESCHERICHIA COLI BY CYTOPLASMIC CHAPERONES CO-EXPRESSION. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 10(3), 141-146. SID. https://sid.ir/paper/313956/en

    Vancouver: Copy

    YOUSEFI MOHAMMAD REZA, FARAJNIA SAFAR, MOKHTARZADEH AHAD, AKBARI BAHMAN, AHDI KHOSROSHAHI SHIVA, MAMIPOUR MINA, DARIUSHNEJAD HASSAN, Ahmadzadeh Vahideh. SOLUBLE EXPRESSION OF HUMANIZED ANTI-CD20 SINGLE CHAIN ANTIBODY IN ESCHERICHIA COLI BY CYTOPLASMIC CHAPERONES CO-EXPRESSION. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2018;10(3):141-146. Available from: https://sid.ir/paper/313956/en

    IEEE: Copy

    MOHAMMAD REZA YOUSEFI, SAFAR FARAJNIA, AHAD MOKHTARZADEH, BAHMAN AKBARI, SHIVA AHDI KHOSROSHAHI, MINA MAMIPOUR, HASSAN DARIUSHNEJAD, and Vahideh Ahmadzadeh, “SOLUBLE EXPRESSION OF HUMANIZED ANTI-CD20 SINGLE CHAIN ANTIBODY IN ESCHERICHIA COLI BY CYTOPLASMIC CHAPERONES CO-EXPRESSION,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 10, no. 3, pp. 141–146, 2018, [Online]. Available: https://sid.ir/paper/313956/en

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