SHORT COMMUNICATION: ASSESSMENT OF EGFR GENE EXPRESSION FOLLOWING VITRIFICATION OF 2-CELL AND BLASTOCYST MOUSE EMBRYOS

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GAZOR ROUHOLLAH; ESKANDARI MOZHGAN; SHARAFSHAH ALIREZA; BAHADORI MOHAMMAD HADI; GOLMOHAMMADI MOHAMMAD GHASEM; KESHAVARZ PARVANEH

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Journal: Avicenna Journal of Medical Biotechnology Year:2018 | Volume:10 | Issue:2 Page(s): 120-122

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Title

SHORT COMMUNICATION: ASSESSMENT OF EGFR GENE EXPRESSION FOLLOWING VITRIFICATION OF 2-CELL AND BLASTOCYST MOUSE EMBRYOS

Author(s)

Keywords

BLASTOCYST

CRYOPRESERVATION

EMBRYO

GENE EXPRESSION

VITRIFICATION

Abstract

Background: Exact mechanisms of fetal harm following VITRIFICATION are still unknown.This study was conducted to evaluate the CRYOPRESERVATION impact on the expression of EpidermalGrowth Factor Receptor (EGFR) gene in mouse 2-cell and BLASTOCYSTs.Methods: To stimulate ovulation in mice, hCG was injected, followed by collecting 2-cells and BLASTOCYSTs after 44-46 and 88-89hr, respectively. These EMBRYOs were divided into two case and control groups. The fresh case group was cryopreserved using cryotop and warmed after 4 mounts. Normal 2-cells were selected based on their morphology and their RNA was extracted. Quantitative expression ofEGFR gene in both groups was investigated by applying real time-PCR.Results: The statistical Real-Time (RT) -PCR analyses performed using SPSS revealed that the expression level ofEGFR gene was diminished in the case group compared to the control group.Conclusion: The current study indicated the negative effect of CRYOPRESERVATION on expression amount ofEGFR gene in 2-cell and BLASTOCYST mouse EMBRYOs.

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APA: Copy

GAZOR, ROUHOLLAH, ESKANDARI, MOZHGAN, SHARAFSHAH, ALIREZA, BAHADORI, MOHAMMAD HADI, GOLMOHAMMADI, MOHAMMAD GHASEM, & KESHAVARZ, PARVANEH. (2018). SHORT COMMUNICATION: ASSESSMENT OF EGFR GENE EXPRESSION FOLLOWING VITRIFICATION OF 2-CELL AND BLASTOCYST MOUSE EMBRYOS. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 10(2), 120-122. SID. https://sid.ir/paper/313965/en

Vancouver: Copy

GAZOR ROUHOLLAH, ESKANDARI MOZHGAN, SHARAFSHAH ALIREZA, BAHADORI MOHAMMAD HADI, GOLMOHAMMADI MOHAMMAD GHASEM, KESHAVARZ PARVANEH. SHORT COMMUNICATION: ASSESSMENT OF EGFR GENE EXPRESSION FOLLOWING VITRIFICATION OF 2-CELL AND BLASTOCYST MOUSE EMBRYOS. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2018;10(2):120-122. Available from: https://sid.ir/paper/313965/en

IEEE: Copy

ROUHOLLAH GAZOR, MOZHGAN ESKANDARI, ALIREZA SHARAFSHAH, MOHAMMAD HADI BAHADORI, MOHAMMAD GHASEM GOLMOHAMMADI, and PARVANEH KESHAVARZ, “SHORT COMMUNICATION: ASSESSMENT OF EGFR GENE EXPRESSION FOLLOWING VITRIFICATION OF 2-CELL AND BLASTOCYST MOUSE EMBRYOS,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 10, no. 2, pp. 120–122, 2018, [Online]. Available: https://sid.ir/paper/313965/en

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