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Information Journal Paper

Title

EVALUATION OF IGY ANTIBODY AS A POLYSPECIFIC COOMBS-REAGENT

Pages

  87-93

Abstract

 Background: During the last twenty years, the extraction of specific egg yolk (IgY) antibodies from the immunized CHICKENs has been accepted as a useful alternative to the IMMUNIZATION of mammals. The aim of the present study was immunizing the CHICKENs with Human Umbilical Cord Serum (HUCS) and the extraction of specific anti-human globulins (IgG, C3b, and C3d) antibodies from egg yolk in order to obtain polyspecific Coombs reagent.Methods: The novelty of this work was the achievement of a polyclonal reagent through a very cheap alternative method in accordance with all ethical regulations required for obtaining it. Three Leghorn hens (21 weeks old) were immunized four times for a period of 66 days with 20uL of HUCS mixed with PBS/FCA or FIA each time. The extraction of IgY antibodies was performed according to the method of lipid precipitation of yolk and using water soluble fraction as the reagent material. The resulting IgY antibody was characterized by SDS-PAGE and immunoelectrophoresis and tested for the presence of hetero-agglutinins by means of direct agglutination using human erythrocytes of all blood groups treated with 0.1% papain and for indirect Coombs-test to evaluate its specificity to fractions (C3b, C3d, C4d) of human complement and human IgG, respectively.Results: Our findings show, that, the reagent obtained contains IgY and other 3 proteins (SDS-PAGE), and reacts specifically with plasma proteins, that migrate inβ andϒregions. In immunoelectrophoresis, in addition, there is the presence of low heteroagglutinins levels in IgY-preparation (3 lots), and the possibility to produce high amount (more than 500ml/egg) of polyspecific Coombs-reagent in CHICKENs is also discussed.Conclusion: IgY-preparation (3 lots), and the possibility to produce high amount (more than 500ml/egg) of polyspecific Coombs-reagent in CHICKENs with the originality to achieve a polyclonal reagent through a very cheap alternative method in accordance with all ethical regulations required for obtaining it, was also discussed.

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    APA: Copy

    GUTIERREZ CALZADO, ESTEBAN JUSTO, TOLEDANO HEREDIA, MARLENE, DUHARTE, JEORGE FERNADEZ, & ZARNANI, AMIR HASSAN. (2017). EVALUATION OF IGY ANTIBODY AS A POLYSPECIFIC COOMBS-REAGENT. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 9(2), 87-93. SID. https://sid.ir/paper/313987/en

    Vancouver: Copy

    GUTIERREZ CALZADO ESTEBAN JUSTO, TOLEDANO HEREDIA MARLENE, DUHARTE JEORGE FERNADEZ, ZARNANI AMIR HASSAN. EVALUATION OF IGY ANTIBODY AS A POLYSPECIFIC COOMBS-REAGENT. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2017;9(2):87-93. Available from: https://sid.ir/paper/313987/en

    IEEE: Copy

    ESTEBAN JUSTO GUTIERREZ CALZADO, MARLENE TOLEDANO HEREDIA, JEORGE FERNADEZ DUHARTE, and AMIR HASSAN ZARNANI, “EVALUATION OF IGY ANTIBODY AS A POLYSPECIFIC COOMBS-REAGENT,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 9, no. 2, pp. 87–93, 2017, [Online]. Available: https://sid.ir/paper/313987/en

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