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Information Journal Paper

Title

Phylogenetic Analysis of Hepatitis B Virus among Household Members with HBV Chronic Infection

Pages

  221-228

Abstract

 Background: Intrafamilial spread of Hepatitis B virus (HBV) infection in Iran has only been investigated with serological testing without using molecular studies as the most informative and definitive type of analysis. Methods: In the present study, intrafamilial Transmission of HBV among family mem-bers of Iranian index HBsAg carriers was investigated using Phylogenetic analysis of the S region of the viral genome. Nested polymerase chain reaction was used for de-tection of HBV DNA in serum samples from 22 index and 43 contact patients with chronic HBV infection. HBV DNA was detected in 37 samples (14 indexes, 23 contacts). The S gene region of the DNA isolates was subjected to direct sequencing and phylo-genetic analysis by Bioedit, Mega and Phylip programs. Results: All isolates (from 26 patients) were clustered with Genotype D, of which 24 strains were of subGenotype D1, subtype ayw2, while 2 additional strains were of sub-Genotype D2, subtype ayw3. Evidence of intrafamilial Transmission of the virus was found in 8 families studied phylogenetically. Overall, 60 changes were detected in the amino acid sequences of the Surface antigen protein in 23 patients. Four premature stop codons occurred in 3 isolates at residues 69 and 182. Seven out of 8 families dis-played 25− 100% common amino acid substitutions among their members. Conclusion: Our data corroborated intrafamilial Transmission of HBV, as evidenced by concordant HBV Genotype among household members, viral sequence homology and close genetic relatedness of the strains on the phylogenetic tree, and horizontal trans-mission of S gene mutations among family members.

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    Cite

    APA: Copy

    SALI, SHAHNAZ, Azarmmanesh, Shirin, Ghalikhani, Hediyeh, & VAEZJALALI, MARYAM. (2019). Phylogenetic Analysis of Hepatitis B Virus among Household Members with HBV Chronic Infection. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 11(3), 221-228. SID. https://sid.ir/paper/314034/en

    Vancouver: Copy

    SALI SHAHNAZ, Azarmmanesh Shirin, Ghalikhani Hediyeh, VAEZJALALI MARYAM. Phylogenetic Analysis of Hepatitis B Virus among Household Members with HBV Chronic Infection. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2019;11(3):221-228. Available from: https://sid.ir/paper/314034/en

    IEEE: Copy

    SHAHNAZ SALI, Shirin Azarmmanesh, Hediyeh Ghalikhani, and MARYAM VAEZJALALI, “Phylogenetic Analysis of Hepatitis B Virus among Household Members with HBV Chronic Infection,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 11, no. 3, pp. 221–228, 2019, [Online]. Available: https://sid.ir/paper/314034/en

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