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Information Journal Paper

Title

Effect of Recombinant Helicobacter Outer Membrane Protein H (HopH) on Nitric Oxide Production by Peripheral Macrophage in BALB/c Mice

Pages

  229-233

Abstract

 Background: Some products of bacteria are reported as an immunomodulator. The Helicobacter pylori (H. pylori) outer membrane proteins play an important role in stimulation of immune system. The present study was performed to determine the in vitro effect of Recombinant HopH of H. pylori on Nitric Oxide (NO) production and viability of mouse peritoneal Macrophages. Methods: H. pylori Recombinant HopH was produced in this study. Mice peritoneal Macrophages were purified and cultured. Different concentrations of Recombinant HopH were used for stimulation of Macrophages in order to evaluate NO production. The cell viability was detected by MTT assay. NO amounts released in to the superna-tants of cultured Macrophages and LPS-stimulated Macrophages (10 μ g/ml) were de-tected by Griess reagent. Results: Results demonstrated that the suppressive effect of high concentrations of re-combinant HopH on NO release and the stimulation effect of protein was shown in 15 μ g/ml, compared to the control group. NO stimulation was significant in all the con-centrations of LPS stimulated with HopH groups. Conclusion: According to our findings, Recombinant HopH has a toxic effect in high concentration on cell. So it can be an anticancer candidate.

Cites

References

Cite

APA: Copy

NAVIDINIA, MASOUMEH, SOLEIMANI, NEDA, & BODAGHABADI, NARGES. (2019). Effect of Recombinant Helicobacter Outer Membrane Protein H (HopH) on Nitric Oxide Production by Peripheral Macrophage in BALB/c Mice. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 11(3), 229-233. SID. https://sid.ir/paper/314038/en

Vancouver: Copy

NAVIDINIA MASOUMEH, SOLEIMANI NEDA, BODAGHABADI NARGES. Effect of Recombinant Helicobacter Outer Membrane Protein H (HopH) on Nitric Oxide Production by Peripheral Macrophage in BALB/c Mice. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2019;11(3):229-233. Available from: https://sid.ir/paper/314038/en

IEEE: Copy

MASOUMEH NAVIDINIA, NEDA SOLEIMANI, and NARGES BODAGHABADI, “Effect of Recombinant Helicobacter Outer Membrane Protein H (HopH) on Nitric Oxide Production by Peripheral Macrophage in BALB/c Mice,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 11, no. 3, pp. 229–233, 2019, [Online]. Available: https://sid.ir/paper/314038/en

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