مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

234
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

162
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Sericin Ameliorates the Capacitation State and Chromatin Integrity of Frozen-Thawed Stallion Spermatozoa by Reducing Oxidative Stress

Pages

  245-252

Abstract

 Background: In the process of sperm cryopreservation, apart from cryoinjury, the production of Reactive Oxygen Species (ROS) can adversely affect the integrity of chromatin and cellular membranes. Addition of natural Antioxidants to freezing me-dium is an approach to reduce the destructive effects of ROS on sperm. Methods: In this study, during 60 min of cooling process, the ejaculates of five stallions were diluted in the following media: INRA 82 medium as Control (C), INRA 82 medi-um supplemented with 0. 25% Sericin (S), INRA 82 medium supplemented with 1. 5 mM Glutathione (G), and INRA 82 medium supplemented with 0. 25% Sericin+1. 5 mM Glutathione (S+G). Results: In the frozen/thawed sericin supplemented group, while the integrity of DNA and the activity of catalase and Glutathione Peroxidase (GPx) were increased, the li-pid peroxidation and midpieceab normality decreased, compared with other groups (p<0. 05). The proportions of sperms with abnormal head in group S and the sperm with distal droplet in G and S+G groups decreased, compared with group C (p<0. 05). In CTC assay, the percentage of capacitated Spermatozoa in treatment groups was lower than control (p<0. 01). Conclusion: In conclusion, the presence of sericin in freezing medium of stallion semen could improve sperm DNA integrity and its resistance to ROS and lipid peroxidation.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    Heidari Nasirabadi, Mahboobeh, SHIRAZI, ABOLFAZL, KADIVAR, ALI, SHAMS ESFANDABADI, NASER, Mohebbi, Abdolnaser, & AHMADI, EBRAHIM. (2019). Sericin Ameliorates the Capacitation State and Chromatin Integrity of Frozen-Thawed Stallion Spermatozoa by Reducing Oxidative Stress. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 11(3), 245-252. SID. https://sid.ir/paper/314043/en

    Vancouver: Copy

    Heidari Nasirabadi Mahboobeh, SHIRAZI ABOLFAZL, KADIVAR ALI, SHAMS ESFANDABADI NASER, Mohebbi Abdolnaser, AHMADI EBRAHIM. Sericin Ameliorates the Capacitation State and Chromatin Integrity of Frozen-Thawed Stallion Spermatozoa by Reducing Oxidative Stress. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2019;11(3):245-252. Available from: https://sid.ir/paper/314043/en

    IEEE: Copy

    Mahboobeh Heidari Nasirabadi, ABOLFAZL SHIRAZI, ALI KADIVAR, NASER SHAMS ESFANDABADI, Abdolnaser Mohebbi, and EBRAHIM AHMADI, “Sericin Ameliorates the Capacitation State and Chromatin Integrity of Frozen-Thawed Stallion Spermatozoa by Reducing Oxidative Stress,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 11, no. 3, pp. 245–252, 2019, [Online]. Available: https://sid.ir/paper/314043/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button