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Information Journal Paper

Title

Expression of Human Placenta-specific 1 (PLAC1) in CHO-K1 Cells

Pages

  24-31

Abstract

 Background: Placenta-specific 1 (PLAC1), as a new Cancer/Testis Antigen (CTA), is frequently expressed in a variety of cancers and localized to cytoplasm and plasma membrane. Surface expression of cancer target antigens is of great importance that enables antibody-mediated cancer immunotherapy. The aim of the current study was to express the intact human PLAC1 protein on plasma membrane of a eukaryotic cell as a model for future anti-PLAC1-based cancer immunotherapy. Methods: In the first approach, entire human PLAC1 gene including its own Signal Peptide (SP) was cloned into pIRES2-EGFP and LeGO-iG2 vectors and expressed in CHO-K1 cells. In the second approach, cytosolic and Signal-Anchor (SA) sequence of Transferrin Receptor Protein 1 (TFR1) were fused to extracellular portion of PLAC1 and expressed as above. Expression of PLAC1 was then assessed using Reverse Transcription Polymerase Chain Reaction (RT-PCR), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF) and Flow Cytometry (FC). Results: The first approach resulted in the expression of PLAC1 in submembranous but not in the surface of transfected CHO-K1 cells. Using the chimeric human PLAC1 construct, the same intracellular expression pattern was observed. Conclusion: These results indicated that there are some yet unknown PLAC1 localization signals employed by cancer cells for surface expression of PLAC1.

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    Cite

    APA: Copy

    MAHMOUDIAN, JAFAR, NAZARI, MAHBOOBEH, GHODS, ROYA, JEDDI TEHRANI, MAHMOOD, OSTAD, SEYED NASSER, GHAHREMANI, MOHAMMAD HOSSEIN, VAFAEI, SEDIGHEH, AMIRI, MOHAMMAD MEHDI, & ZARNANI, AMIR HASSAN. (2020). Expression of Human Placenta-specific 1 (PLAC1) in CHO-K1 Cells. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 12(1), 24-31. SID. https://sid.ir/paper/314065/en

    Vancouver: Copy

    MAHMOUDIAN JAFAR, NAZARI MAHBOOBEH, GHODS ROYA, JEDDI TEHRANI MAHMOOD, OSTAD SEYED NASSER, GHAHREMANI MOHAMMAD HOSSEIN, VAFAEI SEDIGHEH, AMIRI MOHAMMAD MEHDI, ZARNANI AMIR HASSAN. Expression of Human Placenta-specific 1 (PLAC1) in CHO-K1 Cells. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2020;12(1):24-31. Available from: https://sid.ir/paper/314065/en

    IEEE: Copy

    JAFAR MAHMOUDIAN, MAHBOOBEH NAZARI, ROYA GHODS, MAHMOOD JEDDI TEHRANI, SEYED NASSER OSTAD, MOHAMMAD HOSSEIN GHAHREMANI, SEDIGHEH VAFAEI, MOHAMMAD MEHDI AMIRI, and AMIR HASSAN ZARNANI, “Expression of Human Placenta-specific 1 (PLAC1) in CHO-K1 Cells,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 12, no. 1, pp. 24–31, 2020, [Online]. Available: https://sid.ir/paper/314065/en

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