EVALUATION OF PATHOGENIC SEROVARS OF LEPTOSPIRA INTERROGANS IN DAIRY CATTLE HERDS OF SHAHREKORD BY PCR

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

JAFARI DEHKORDI A.; SHAHBAZKIA H.R.; RONAGH N.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Microbiology Year:2011 | Volume:3 | Issue:3 Page(s): 135-139

Download Full-Text

View:

251

Download:

Cites:

Information Journal Paper

Title

EVALUATION OF PATHOGENIC SEROVARS OF LEPTOSPIRA INTERROGANS IN DAIRY CATTLE HERDS OF SHAHREKORD BY PCR

Author(s)

JAFARI DEHKORDI A. | SHAHBAZKIA H.R. | RONAGH N. | Issue Writer Certificate

Keywords

LEPTOSPIRA

SHAHREKORD

CATTLE

PCR

Abstract

Background and objectives: Leptospirosis is an important zoonotic disease caused by LEPTOSPIRA interrogans. Leptospirosis leads to economical losses in dairy farm industry. The objective of this study was to evaluate the pathogenic serovars of LEPTOSPIRA interrogans in dairy CATTLE herds of SHAHREKORD by PCR.Materials and Methods: Two hundred samples (100 urine and 100 blood) were collected from 100 cows randomly and delivered to the laboratory. Samples were stored at -20oC. DNA was extracted and purified from the plasma and urine samples and concentrated on diatoms in the presence of guanidine thiocyanate (GuSCN). PCR products were detected and identified as LEPTOSPIRA by ilumination of the expected size of DNA bands after staining of the agarose gel with ethidium bromide gels. PCR products were purified and sequenced.Results: The results showed that 28% of urine samples and 23% of plasma samples were contaminated. The major serotypes were Icterohaemorrhagiae (50%) and Pomona (37.5%). The urine samples of 17 cows were positive for LEPTOSPIRA without positive plasma samples. This indicated that these cows are reservoirs in dairy herds of SHAHREKORD and dangerous for human health. The plasma samples of twelve cows were positive for LEPTOSPIRA without positive urine samples.Conclusions: LEPTOSPIRA serotypes can be maintained in relatively dry regions and must be considered when dealing with leptospirosis in dairy farms of SHAHREKORD and human health.

Cites

No record.

References

No record.

Cite

APA: Copy

JAFARI DEHKORDI, A., SHAHBAZKIA, H.R., & RONAGH, N.. (2011). EVALUATION OF PATHOGENIC SEROVARS OF LEPTOSPIRA INTERROGANS IN DAIRY CATTLE HERDS OF SHAHREKORD BY PCR. IRANIAN JOURNAL OF MICROBIOLOGY, 3(3), 135-139. SID. https://sid.ir/paper/315289/en

Vancouver: Copy

JAFARI DEHKORDI A., SHAHBAZKIA H.R., RONAGH N.. EVALUATION OF PATHOGENIC SEROVARS OF LEPTOSPIRA INTERROGANS IN DAIRY CATTLE HERDS OF SHAHREKORD BY PCR. IRANIAN JOURNAL OF MICROBIOLOGY[Internet]. 2011;3(3):135-139. Available from: https://sid.ir/paper/315289/en

IEEE: Copy

A. JAFARI DEHKORDI, H.R. SHAHBAZKIA, and N. RONAGH, “EVALUATION OF PATHOGENIC SEROVARS OF LEPTOSPIRA INTERROGANS IN DAIRY CATTLE HERDS OF SHAHREKORD BY PCR,” IRANIAN JOURNAL OF MICROBIOLOGY, vol. 3, no. 3, pp. 135–139, 2011, [Online]. Available: https://sid.ir/paper/315289/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops