مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

422
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

80
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

3

Information Journal Paper

Title

MOLECULAR IDENTIFICATION AND DETECTION OF VIRULENCE GENES AMONG PSEUDOMONAS AERUGINOSAISOLATED FROM DIFFERENT INFECTIOUS ORIGINS

Pages

  118-123

Abstract

 Background and Objectives: PSEUDOMONAS AERUGINOSA possesses a variety of VIRULENCE FACTORS that may contribute to its pathogenicity. The aim of this study was to evaluate opr I, oprL and toxA genes for PCR identification of clinical P. aeruginosa. In order to find out any relation between special VIRULENCE FACTORS and special manifestation of P. aeruginosa infections, we detected VIRULENCE FACTORS among these isolates by PCR. RIBOTYPING was used to evaluate the clonal relationship between strains with the same genetic patterns of the genes studied.Materials and Methods: In this study, 268 isolates of P. aeruginosa were recovered from burn, wound and pulmonary tract infections. The prevalence of oprI, oprL, toxA, lasB, exoS and nan1 genes was determined by PCR. One hundred and four isolates were selected randomly to investigate clonal diversity of the isolates with RIBOTYPING using Sma I.Results and Conclusions: All P. aeruginosa isolates in this study carried oprI, oprL and lasB genes. Difference between exo Sprevalence in isolates from pulmonary tract and burn isolates was statistically significant. Prevalence of nan1 and toxA gene was significantly higher in pulmonary tract and burn isolates, respectively. RIBOTYPING showed that most of the isolates (87%) belonged to clone A and B. Detection of oprI, oprL and tox A genes by PCR is recommended for molecular identification of P. aeruginosa. Determination of different virulence genes of P. aeruginosa isolates suggests that they are associated with different levels of intrinsic virulence and pathogenicity. RIBOTYPING showed that strains with the same genetic patterns of the genes do not necessarily have similar ribotype patterns.

Cites

References

  • No record.

    • Cite

    APA: Copy

    NIKBIN, V.S., ASLANI, MOHAMMAD MEHDI, SHARAFI, Z., HASHEMI POUR, M., SHAH CHERAGHI, F., & EBRAHIMIPOUR, GH.. (2012). MOLECULAR IDENTIFICATION AND DETECTION OF VIRULENCE GENES AMONG PSEUDOMONAS AERUGINOSAISOLATED FROM DIFFERENT INFECTIOUS ORIGINS. IRANIAN JOURNAL OF MICROBIOLOGY, 4(3), 118-123. SID. https://sid.ir/paper/315323/en

    Vancouver: Copy

    NIKBIN V.S., ASLANI MOHAMMAD MEHDI, SHARAFI Z., HASHEMI POUR M., SHAH CHERAGHI F., EBRAHIMIPOUR GH.. MOLECULAR IDENTIFICATION AND DETECTION OF VIRULENCE GENES AMONG PSEUDOMONAS AERUGINOSAISOLATED FROM DIFFERENT INFECTIOUS ORIGINS. IRANIAN JOURNAL OF MICROBIOLOGY[Internet]. 2012;4(3):118-123. Available from: https://sid.ir/paper/315323/en

    IEEE: Copy

    V.S. NIKBIN, MOHAMMAD MEHDI ASLANI, Z. SHARAFI, M. HASHEMI POUR, F. SHAH CHERAGHI, and GH. EBRAHIMIPOUR, “MOLECULAR IDENTIFICATION AND DETECTION OF VIRULENCE GENES AMONG PSEUDOMONAS AERUGINOSAISOLATED FROM DIFFERENT INFECTIOUS ORIGINS,” IRANIAN JOURNAL OF MICROBIOLOGY, vol. 4, no. 3, pp. 118–123, 2012, [Online]. Available: https://sid.ir/paper/315323/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button