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Information Journal Paper

Title

A BIOASSAY-GUIDED FRACTIONATION SCHEME FOR CHARACTERIZATION OF NEW ANTIBACTERIAL COMPOUNDS FROM PROSOPIS CINERARIA AERIAL PARTS

Pages

  1-7

Abstract

 Background and Objectives: Due to the importance of finding of new antibacterial agents, the antibacterial properties of PROSOPIS CINERARIA aerial parts were investigated using a BIOASSAY guided fractionation scheme.Materials and Methods: The organic extract was prepared via maceration in methanol, followed by the fractionation using n-hexane and ethyl acetate. The MICs of fractions were determined against some human pathogenic bacteria using broth micro-dilution assay. The primary CHARACTERIZATION and identification of bioactive substance(s) was based on a bio-autographical method using HPTLC and flash chromatography in parallel with agar overlay assays. Finally the exact mass of effective compound(s) was determined by LC-MS.Results: The best antibacterial activities were related to the ethyl acetate fraction. The effective antibacterial compound of the plant were 2 substances with molecular weight of 348 and 184 Dalton that inhibited the growth of assessed Gram positive bacteria with MIC values lower than 125 to 62.5 μg/ml synergistically.Conclusion: Further analysis using nuclear magnetic resonance could reveal the exact structure of these two antibacterial substances. These 2 effective antibacterial compounds could be applied as lead compound for synthesis of new antibacterial agents.

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    APA: Copy

    NEGHABI HAJIAGHA, MAHDIEH, ALIAHMADI, ATOUSA, TAHERI, MOHAMMADREZA, GHASSEMPOUR, ALIREZA, IRAJIAN, GHOLAMREZA, REZADOOST, HASSAN, & FEIZABADI, MOHAMMADMEHDI. (2016). A BIOASSAY-GUIDED FRACTIONATION SCHEME FOR CHARACTERIZATION OF NEW ANTIBACTERIAL COMPOUNDS FROM PROSOPIS CINERARIA AERIAL PARTS. IRANIAN JOURNAL OF MICROBIOLOGY, 8(1), 1-7. SID. https://sid.ir/paper/315390/en

    Vancouver: Copy

    NEGHABI HAJIAGHA MAHDIEH, ALIAHMADI ATOUSA, TAHERI MOHAMMADREZA, GHASSEMPOUR ALIREZA, IRAJIAN GHOLAMREZA, REZADOOST HASSAN, FEIZABADI MOHAMMADMEHDI. A BIOASSAY-GUIDED FRACTIONATION SCHEME FOR CHARACTERIZATION OF NEW ANTIBACTERIAL COMPOUNDS FROM PROSOPIS CINERARIA AERIAL PARTS. IRANIAN JOURNAL OF MICROBIOLOGY[Internet]. 2016;8(1):1-7. Available from: https://sid.ir/paper/315390/en

    IEEE: Copy

    MAHDIEH NEGHABI HAJIAGHA, ATOUSA ALIAHMADI, MOHAMMADREZA TAHERI, ALIREZA GHASSEMPOUR, GHOLAMREZA IRAJIAN, HASSAN REZADOOST, and MOHAMMADMEHDI FEIZABADI, “A BIOASSAY-GUIDED FRACTIONATION SCHEME FOR CHARACTERIZATION OF NEW ANTIBACTERIAL COMPOUNDS FROM PROSOPIS CINERARIA AERIAL PARTS,” IRANIAN JOURNAL OF MICROBIOLOGY, vol. 8, no. 1, pp. 1–7, 2016, [Online]. Available: https://sid.ir/paper/315390/en

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