FREQUENCY EVALUATION OF GENES ENCODING SIDEROPHORES AND THE EFFECTS OF DIFFERENT CONCENTRATIONS OF FE IONS ON GROWTH RATE OF UROPATHOGENICESCHERICHIA COLI

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KHASHEII BEHNOUSH; ANVARI SHAGHAYEGH; JAMALLI AILAR

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Journal: Iranian Journal of Microbiology Year:2016 | Volume:8 | Issue:6 Page(s): 359-365

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Title

FREQUENCY EVALUATION OF GENES ENCODING SIDEROPHORES AND THE EFFECTS OF DIFFERENT CONCENTRATIONS OF FE IONS ON GROWTH RATE OF UROPATHOGENICESCHERICHIA COLI

Author(s)

KHASHEII BEHNOUSH | ANVARI SHAGHAYEGH | JAMALLI AILAR | Issue Writer Certificate

Keywords

KEYWORDS: ESCHERICHIA COLI

SIDEROPHORE

AEROBACTIN

SALMOCHELIN

YERSINIABACTIN

FE (II) SUPPLEMENTS

Abstract

Background and Objectives: Bacteria need iron for growth and most of them can actively acquire Fe ions using especialiron-chelating proteins which named SIDEROPHOREs. We aimed to determine the frequencies of iucA, iroN and irp2 genes in theuropathogenic Escherichia coli (UPEC) isolates. We also analyzed the effects of SIDEROPHORE genes beside iron supplementson growth rate of the isolates.Materials and Methods: Totally, 170 E. coli strains were isolated from urinary tract infections and the presence of 3 SIDEROPHOREgenes were analyzed using PCR among them. Three final concentrations of 0.1, 0.5 and 1 mMFe (II) and Fe (III)ions were made in M9 broth medium. Inoculated cultures were incubated at 37°C for 33 hours and bacterial density in thesuspension was measured with 1 hour intervals using spectrophotometer.Results: The frequency of iucA, iroN and irp2 genes among 170 UPEC isolates were 29 (17.1%), 52 (30.6%) and 116(68.2%), respectively. In addition, Our findings showed that Fe (II) supplements had significantly higher promoting effectson UPEC growth rate almost in all of the three applied concentrations (0.1, 0.5 and 1 mM) compared to the control group(P<0.0001). Differences between Fe (III) supplemented groups and the controls were statistically significant when 1 mMconcentration was added into the medium (p<0.05).Conclusion: irp2 gene probably plays a major role in the pathogenesis of UPEC strains. Promoting or inhibitory effects ofiron on bacterial growth mainly depend on the iron concentration in the culture medium however different SIDEROPHOREs havedifferent potentials for capturing and assimilation of Fe ions by the bacteria, especially inside the host cell.

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APA: Copy

KHASHEII, BEHNOUSH, ANVARI, SHAGHAYEGH, & JAMALLI, AILAR. (2016). FREQUENCY EVALUATION OF GENES ENCODING SIDEROPHORES AND THE EFFECTS OF DIFFERENT CONCENTRATIONS OF FE IONS ON GROWTH RATE OF UROPATHOGENICESCHERICHIA COLI. IRANIAN JOURNAL OF MICROBIOLOGY, 8(6), 359-365. SID. https://sid.ir/paper/315444/en

Vancouver: Copy

KHASHEII BEHNOUSH, ANVARI SHAGHAYEGH, JAMALLI AILAR. FREQUENCY EVALUATION OF GENES ENCODING SIDEROPHORES AND THE EFFECTS OF DIFFERENT CONCENTRATIONS OF FE IONS ON GROWTH RATE OF UROPATHOGENICESCHERICHIA COLI. IRANIAN JOURNAL OF MICROBIOLOGY[Internet]. 2016;8(6):359-365. Available from: https://sid.ir/paper/315444/en

IEEE: Copy

BEHNOUSH KHASHEII, SHAGHAYEGH ANVARI, and AILAR JAMALLI, “FREQUENCY EVALUATION OF GENES ENCODING SIDEROPHORES AND THE EFFECTS OF DIFFERENT CONCENTRATIONS OF FE IONS ON GROWTH RATE OF UROPATHOGENICESCHERICHIA COLI,” IRANIAN JOURNAL OF MICROBIOLOGY, vol. 8, no. 6, pp. 359–365, 2016, [Online]. Available: https://sid.ir/paper/315444/en

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