Expression and purification of recombinant HTLV-I/-II linear epitopes antigen and its application for screening of suspected patients

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Faramarzi Roxana; Dolatabadi Samaneh

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Journal Paper

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Journal: Iranian Journal of Microbiology Year:2017 | Volume:9 | Issue:1 Page(s): 43-49

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Title

Expression and purification of recombinant HTLV-I/-II linear epitopes antigen and its application for screening of suspected patients

Author(s)

Faramarzi Roxana | Dolatabadi Samaneh | Issue Writer Certificate

Keywords

HTLV

Chimeric antigen

Protein expression

Purification

Abstract

Background and Objectives: The linear epitopes of gp46-I, gp46-II, gp21 and p19 are used in diagnosis of HTLV-I/-II infections. The aims of this study was to obtain high-level expression and Purification of recombinant antigen (RA) containing these epitopes. Large-scale preparation of such antigen probably worths for diagnostic purpose. Materials and Methods: The synthetic DNA encoding RA was synthesized and over-expressed as soluble in Escherichia coli BL21 (DE3) cells. Expression and distribution of the His-GST-RA protein were evaluated using SDS-PAGE. The soluble RA was purified utilizing Ni-NTA agarose beads under native conditions and was concentrated by ultra filtration. Using 20 sera specimens from HTLV infected patients, the antigenicity of the purified protein was confirmed in ELISA and western blotting analysis. Results: SDS-PAGE revealed that the purified protein was more than 90% pure. The final yield was approximately 25 mg per liter of culture medium. ELISA results showed that RA could specifically bind to anti-HTLV-I/-II antibodies in infected sera. Conclusion: RA could be a candidate for HTLV-I/-II screening and the strategy presented in this study could be used for easy production of this diagnostic protein.

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APA: Copy

Faramarzi, Roxana, & Dolatabadi, Samaneh. (2017). Expression and purification of recombinant HTLV-I/-II linear epitopes antigen and its application for screening of suspected patients. IRANIAN JOURNAL OF MICROBIOLOGY, 9(1), 43-49. SID. https://sid.ir/paper/315492/en

Vancouver: Copy

Faramarzi Roxana, Dolatabadi Samaneh. Expression and purification of recombinant HTLV-I/-II linear epitopes antigen and its application for screening of suspected patients. IRANIAN JOURNAL OF MICROBIOLOGY[Internet]. 2017;9(1):43-49. Available from: https://sid.ir/paper/315492/en

IEEE: Copy

Roxana Faramarzi, and Samaneh Dolatabadi, “Expression and purification of recombinant HTLV-I/-II linear epitopes antigen and its application for screening of suspected patients,” IRANIAN JOURNAL OF MICROBIOLOGY, vol. 9, no. 1, pp. 43–49, 2017, [Online]. Available: https://sid.ir/paper/315492/en

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