Isolation and identification of Komagataeibacter xylinus from Iranian traditional vinegars and molecular analyses

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Lavasani Paria Sadat; MOTEVASELI ELAHE; SHIRZAD MAHDIEH; MODARRESSI MOHAMMAD HOSSEIN

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Journal Paper

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Journal: Iranian Journal of Microbiology Year:2017 | Volume:9 | Issue:6 Page(s): 338-347

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Title

Isolation and identification of Komagataeibacter xylinus from Iranian traditional vinegars and molecular analyses

Author(s)

Lavasani Paria Sadat | MOTEVASELI ELAHE | SHIRZAD MAHDIEH | MODARRESSI MOHAMMAD HOSSEIN | Issue Writer Certificate

Keywords

Komagataeibacter xylinus

16S rRNA

bcsA

pheS

rpoA

Abstract

Background and Objectives: Acetic acid bacteria (AAB) are one of the major interests of researchers. Traditional vinegars are suitable sources of AAB because they are not undergone industrial process like filtering and adding preservatives. Komagataeibacter xylinus as a member of AAB is known as the main cellulose producer among other bacteria. The purpose of the current study was to isolate the bacteria from traditional vinegars and its molecular analyses. Materials and Methods: Vinegar samples were collected. Well-organized bacteriological tests were carried out to differentiate isolated bacteria from other cellulose producers and to identify K. xylinus. NaOH treatment and Calcofluor white staining were used for detecting cellulose. Chromosomal DNA of each strain was extracted via three methods of boiling, phenol-chloroform and sonication. Molecular analyses were performed on the basis of 16S rRNA sequences and cellulose synthase catalytic subunit gene (bcsA) for further confirmation. Phylogenetic tree was constructed for more characterization. Two housekeeping genes were studied including phenylalanyl-tRNA synthase alpha subunit (pheS) and RNA polymerase alpha subunit (rpoA). Results: Of the 97 samples, 43 K. xylinus strains were isolated. They were identified via bacteriological and molecular techniques. 16S rDNA sequence showed 99% similarity with registered sequences of the bacteria. Biodiversity of the genome confirmed by analyzing bcsA, pheS and rpoA genes. Conclusion: K. xylinus can be isolated from traditional vinegars. Screening tests ought to include the classical methods and molecular techniques. Different molecular techniques and more genomic research should be developed to expand our knowledge for distinguishing isolated bacteria especially in the fields of AAB.

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APA: Copy

Lavasani, Paria Sadat, MOTEVASELI, ELAHE, SHIRZAD, MAHDIEH, & MODARRESSI, MOHAMMAD HOSSEIN. (2017). Isolation and identification of Komagataeibacter xylinus from Iranian traditional vinegars and molecular analyses. IRANIAN JOURNAL OF MICROBIOLOGY, 9(6), 338-347. SID. https://sid.ir/paper/315530/en

Vancouver: Copy

Lavasani Paria Sadat, MOTEVASELI ELAHE, SHIRZAD MAHDIEH, MODARRESSI MOHAMMAD HOSSEIN. Isolation and identification of Komagataeibacter xylinus from Iranian traditional vinegars and molecular analyses. IRANIAN JOURNAL OF MICROBIOLOGY[Internet]. 2017;9(6):338-347. Available from: https://sid.ir/paper/315530/en

IEEE: Copy

Paria Sadat Lavasani, ELAHE MOTEVASELI, MAHDIEH SHIRZAD, and MOHAMMAD HOSSEIN MODARRESSI, “Isolation and identification of Komagataeibacter xylinus from Iranian traditional vinegars and molecular analyses,” IRANIAN JOURNAL OF MICROBIOLOGY, vol. 9, no. 6, pp. 338–347, 2017, [Online]. Available: https://sid.ir/paper/315530/en

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