CLONE PURIFICATION, CHARACTERIZATION AND STANDARDIZATION OF LASOTA STRAIN FOR DEVELOPING A LIVE VACCINE AGAINST NEWCASTLE DISEASE VIRUS

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EBRAHIMI M.M.; SHAHSAVANDI S.; FAMIL GHADAKCHI H.; MASOUDI S.; GHODSIAN N.; EBRAHIMI S.R.; ABDOSHAH M.

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Paper Information

Journal: IRANIAN JOURNAL OF VIROLOGY Year:2014 | Volume:8 | Issue:4 Page(s): 12-19

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Title

CLONE PURIFICATION, CHARACTERIZATION AND STANDARDIZATION OF LASOTA STRAIN FOR DEVELOPING A LIVE VACCINE AGAINST NEWCASTLE DISEASE VIRUS

Author(s)

Keywords

NEWCASTLE DISEASE VIRUS

LASOTA

CLONE PURIFICATION

LIVE VACCINE

Abstract

Background and Aims: Newcastle disease (ND) is one of the most serious illnesses of chickens. LIVE VACCINEs are widely used to prevent chicken from the disease all over the world.Materials and Methods: To access the effective and potentiate ND vaccine, a homogenous subpopulation from LASOTA strain was selected following cultivation of the virus on primary chicken embryofibroblast (CEF) cells. Pathogenicity indices and molecular characterization of a several plaques at 3rd passage were analyzed and then the selected clone was candidate for vaccine development. ND vaccine was prepared according to the standard protocols.Results: The immunogenicity of the LIVE VACCINE was examined in specific pathogen free (SPF) and commercial chickens. The geometric mean hemagglutination-inhibition (HI) titers induced in chickens vaccinated with the cloned vaccine were not significantly differ than those induced in chickens vaccinated with the similar ND clone vaccine.Conclusion: Efficacy of the ND vaccine was estimated against the virus challenge. The results indicate that the cloned vaccine could confer a complete protection against NDV.

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APA: Copy

EBRAHIMI, M.M., SHAHSAVANDI, S., FAMIL GHADAKCHI, H., MASOUDI, S., GHODSIAN, N., EBRAHIMI, S.R., & ABDOSHAH, M.. (2014). CLONE PURIFICATION, CHARACTERIZATION AND STANDARDIZATION OF LASOTA STRAIN FOR DEVELOPING A LIVE VACCINE AGAINST NEWCASTLE DISEASE VIRUS. IRANIAN JOURNAL OF VIROLOGY, 8(4), 12-19. SID. https://sid.ir/paper/315702/en

Vancouver: Copy

EBRAHIMI M.M., SHAHSAVANDI S., FAMIL GHADAKCHI H., MASOUDI S., GHODSIAN N., EBRAHIMI S.R., ABDOSHAH M.. CLONE PURIFICATION, CHARACTERIZATION AND STANDARDIZATION OF LASOTA STRAIN FOR DEVELOPING A LIVE VACCINE AGAINST NEWCASTLE DISEASE VIRUS. IRANIAN JOURNAL OF VIROLOGY[Internet]. 2014;8(4):12-19. Available from: https://sid.ir/paper/315702/en

IEEE: Copy

M.M. EBRAHIMI, S. SHAHSAVANDI, H. FAMIL GHADAKCHI, S. MASOUDI, N. GHODSIAN, S.R. EBRAHIMI, and M. ABDOSHAH, “CLONE PURIFICATION, CHARACTERIZATION AND STANDARDIZATION OF LASOTA STRAIN FOR DEVELOPING A LIVE VACCINE AGAINST NEWCASTLE DISEASE VIRUS,” IRANIAN JOURNAL OF VIROLOGY, vol. 8, no. 4, pp. 12–19, 2014, [Online]. Available: https://sid.ir/paper/315702/en

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