A STUDY ON GENOTYPES OF HEPATITIS B VIRUS AMONG HEMODIALYSIS PATIENTS IN KHUZESTAN PROVINCE

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NEISI NILOOFAR; MAKVANDI MANOCHEHR; SAMARBAF ZADEH ALI REZA

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Journal Paper

Paper Information

Journal: Jundishapur Journal of Microbiology Year:2011 | Volume:4 | Issue:2 (S.N. 11) Page(s): 65-70

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Title

A STUDY ON GENOTYPES OF HEPATITIS B VIRUS AMONG HEMODIALYSIS PATIENTS IN KHUZESTAN PROVINCE

Author(s)

NEISI NILOOFAR | MAKVANDI MANOCHEHR | SAMARBAF ZADEH ALI REZA | Issue Writer Certificate

Keywords

HEPATITIS B VIRUS (HBV)

HEMODIALYSIS

GENOTYPING

RFLP

Abstract

Introduction and objective: Hepatitis B virus (HBV) is a member of Hepadnaviridae and a major causative agent of chronic and acute hepatitis, liver cirrhosis and hepatocellular carcinoma. Genotype determination of HBV is based on PCR-RFLP and sequencing of genome of the virus. The genotype formation is mainly due to mutations of HBV precore, S, and YMDD (tyrosine-methionine-aspartate-aspartate motif in the C domain of the HBV DNA polymerase gene) genome area. Moreover, some of the mutant HBV remains undetectable by serological tests (occult hepatitis). Since the genotypes of HBV and occult hepatitis B has not been studied in our area, this study was conducted to determine both occult hepatitis B infection and genotypes among HEMODIALYSIS patients.Materials and methods: Two hundred and fifty HEMODIALYSIS patients were selected in this study. The sera of the patients were collected and the extracted DNA was used as template of PCR to amplify a 479bp fragment of the viral genome. The PCR products were digested by Ava2 and Mbo1 restriction enzymes. Based on RFLP patterns, the genotypes were determined. The HBV markers including; HBV surface antigen (HBsAg), hepatitis Bc antibody (HBcIgG), hepatitis B virus e antigen (HBeAg) and hepatitis B virus e antibody (HBeAb) were carried out for all the patients by ELISA test.Results: Fifty (20%) out of 250 sera showed positive HBV by PCR. Out of the 50 positive cases for HBV, 46 (92%) belonged to genotype D2 and 2 (4%) cases of them were B6 genotype. Ten cases were positive for HBV by PCR test but negative by ELISA test (4% occult hepatitis).Conclusion: Prevalence of HBV infection was high among the dialysis patients (20%), and occult hepatitis B was 4% in these patients. The dominant genotype of HBV was D2 (92%) followed by genotypes B6 (4%) in HEMODIALYSIS patients.

Cites

APPROPRIATE GENOTYPING OF HEPATITIS B VIRUS IN IRAN

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APA: Copy

NEISI, NILOOFAR, MAKVANDI, MANOCHEHR, & SAMARBAF ZADEH, ALI REZA. (2011). A STUDY ON GENOTYPES OF HEPATITIS B VIRUS AMONG HEMODIALYSIS PATIENTS IN KHUZESTAN PROVINCE. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 4(2 (S.N. 11)), 65-70. SID. https://sid.ir/paper/317181/en

Vancouver: Copy

NEISI NILOOFAR, MAKVANDI MANOCHEHR, SAMARBAF ZADEH ALI REZA. A STUDY ON GENOTYPES OF HEPATITIS B VIRUS AMONG HEMODIALYSIS PATIENTS IN KHUZESTAN PROVINCE. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2011;4(2 (S.N. 11)):65-70. Available from: https://sid.ir/paper/317181/en

IEEE: Copy

NILOOFAR NEISI, MANOCHEHR MAKVANDI, and ALI REZA SAMARBAF ZADEH, “A STUDY ON GENOTYPES OF HEPATITIS B VIRUS AMONG HEMODIALYSIS PATIENTS IN KHUZESTAN PROVINCE,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 4, no. 2 (S.N. 11), pp. 65–70, 2011, [Online]. Available: https://sid.ir/paper/317181/en

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