BIOLOGICAL AND IMMUNOLOGICAL EVALUATION OF NEISSERIA MENINGITIDIS SEROGROUP A OUTER MEMBRANE VESICLE AS VACCINE CANDIDATES

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DELBAZ SEYED ALI; SIADAT SEYED DAVAR; AGHASADEGHI MOHAMMAD REZA; PIRYAIE MOHAMAD; NAJAR PEERAYEH SHAHIN; MOUSAVI SEYED FAZLOLLAH; MOSHIRI ARFA; SADAT SEYED MEHDI; ZANGENEH MEHRANGIZ; NEJATI MEHDI; KASHANIZADEH NAFISEH; BOUZARI SAIED

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Journal: Jundishapur Journal of Microbiology Year:2013 | Volume:6 | Issue:4 (S.N. 22) Page(s): 1-6

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Title

BIOLOGICAL AND IMMUNOLOGICAL EVALUATION OF NEISSERIA MENINGITIDIS SEROGROUP A OUTER MEMBRANE VESICLE AS VACCINE CANDIDATES

Author(s)

Keywords

OUTER MEMBRANE VESICLE (OMV)

NEISSERIA MENINGITIDIS

SEROGROUP A

VACCINES

Abstract

Background: NEISSERIA MENINGITIDIS SEROGROUP A, is a major cause of bacterial meningitidis outbreaks in Africa and the Middle East. While polysaccharide VACCINES have been available for many years, these VACCINES have many disadvantages including the induction of T-cell independent responses which do not induce memory responses. Objectives: Thus to overcome this problem, in this research outer membrane vesicle (OMV) containing PorA was extracted and evaluated by biological and immunological methods. Materials and Methods: OMVs were extracted with deoxycholate and EDTA, and purification was performed by sequential ultracentrifugation. Physicochemical properties of extracted OMVs were analyzed by electron microscopy and SDS-PAGE. The toxicity of LPS content in its was assayed by LAL test. The Presence of PorA as a major component of OMV was confirmed by western blot. To study antibodies synthesis after immunization with OMV, ELISA method was used. Also serum bactericidal assay (SBA) was performed to determine the serum bactericidal activity against N. meningitidis SEROGROUP A. Results: The results revealed that the content of protein extracted was 0.1 mg/mL. The electron microscopy showed that intactness of the vesicle in these preparation ranged more than 70%. The SDS-PAGE showed that PorA as a major immunological part of outer membrane vesicle was located in 35-40kDa. LAL test showed that the endotoxin activity was around 126EU/mL which is safe for using. The ELISA test revealed that the IgG total titer was elevated after the first injection. SBA indicates that bactericidal antibodies rise after the second dose of booster. Conclusions: The results showed that the extracted OMVs were conformationally stable, and there were no pyrogenic determinants in OMV. Also the results showed that the OMV elicited high level of specific antibodies against N. meningitidis SEROGROUP A. These results indicate that the OMV obtained here, can be used as a meningococcal vaccine after further investigation.

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APA: Copy

DELBAZ, SEYED ALI, SIADAT, SEYED DAVAR, AGHASADEGHI, MOHAMMAD REZA, PIRYAIE, MOHAMAD, NAJAR PEERAYEH, SHAHIN, MOUSAVI, SEYED FAZLOLLAH, MOSHIRI, ARFA, SADAT, SEYED MEHDI, ZANGENEH, MEHRANGIZ, NEJATI, MEHDI, KASHANIZADEH, NAFISEH, & BOUZARI, SAIED. (2013). BIOLOGICAL AND IMMUNOLOGICAL EVALUATION OF NEISSERIA MENINGITIDIS SEROGROUP A OUTER MEMBRANE VESICLE AS VACCINE CANDIDATES. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 6(4 (S.N. 22)), 1-6. SID. https://sid.ir/paper/317332/en

Vancouver: Copy

DELBAZ SEYED ALI, SIADAT SEYED DAVAR, AGHASADEGHI MOHAMMAD REZA, PIRYAIE MOHAMAD, NAJAR PEERAYEH SHAHIN, MOUSAVI SEYED FAZLOLLAH, MOSHIRI ARFA, SADAT SEYED MEHDI, ZANGENEH MEHRANGIZ, NEJATI MEHDI, KASHANIZADEH NAFISEH, BOUZARI SAIED. BIOLOGICAL AND IMMUNOLOGICAL EVALUATION OF NEISSERIA MENINGITIDIS SEROGROUP A OUTER MEMBRANE VESICLE AS VACCINE CANDIDATES. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2013;6(4 (S.N. 22)):1-6. Available from: https://sid.ir/paper/317332/en

IEEE: Copy

SEYED ALI DELBAZ, SEYED DAVAR SIADAT, MOHAMMAD REZA AGHASADEGHI, MOHAMAD PIRYAIE, SHAHIN NAJAR PEERAYEH, SEYED FAZLOLLAH MOUSAVI, ARFA MOSHIRI, SEYED MEHDI SADAT, MEHRANGIZ ZANGENEH, MEHDI NEJATI, NAFISEH KASHANIZADEH, and SAIED BOUZARI, “BIOLOGICAL AND IMMUNOLOGICAL EVALUATION OF NEISSERIA MENINGITIDIS SEROGROUP A OUTER MEMBRANE VESICLE AS VACCINE CANDIDATES,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 6, no. 4 (S.N. 22), pp. 1–6, 2013, [Online]. Available: https://sid.ir/paper/317332/en

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