GENOTYPIC IDENTIFICATION OF AMPC β-LACTAMASES PRODUCTION IN GRAM-NEGATIVE BACILLI ISOLATES

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WASSEF MONA; BEHIRY IMAN; YOUNAN MARIAM; EL GUINDY NANCY; MOSTAFA SALLY; ABADA EMAD

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Journal: Jundishapur Journal of Microbiology Year:2014 | Volume:7 | Issue:1 Page(s): 0-0

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Title

GENOTYPIC IDENTIFICATION OF AMPC β-LACTAMASES PRODUCTION IN GRAM-NEGATIVE BACILLI ISOLATES

Author(s)

Keywords

AMPC β-LACTAMASE

BORONIC ACID

CLOXACILLIN

MULTIPLEX PCR

AMPC GENES

Abstract

Background: AmpC type b-lactamases are commonly isolated from extended-spectrum Cephalosporin-resistant Gram-negative bacteria. Also, resistance appeared in bacterial species not naturally producing AmpC enzymes. Therefore, a standard test for the detection of the plasmid-mediated AmpC enzyme and new breakpoints for extended spectrum Cephalosporins are urgently necessary.Objectives: To detect plasmid and chromosomal mediated AmpC- b-lactamases in Gram negative bacteria in community and hospital acquired infections.Materials and Methods: 1073 Gram negative clinical isolates were identified by the conventional methods and were screened for AmpC production using Cefoxitin discs. Confirmatory phenotypic identifications were done for the Cefoxitin-resistant isolates using BORONIC ACID for combined and double disc synergy tests, CLOXACILLIN based double disc synergy test, and induction tests. The genotypic identification of plasmid-mediated AmpC was done using MULTIPLEX PCR. ESBL production was also screened by discs of Ceftazidime and Cefotaxime with and without Clavulanic Acid (10 mg).Results: The AmpC-producing isolates among all identified Gram negative bacilli were 5.8% (62/1073) as detected by screening disc diffusion methods, where 72% were positive for AmpC by combined disc method (Cefotetan and BORONIC ACID), 56.5% were positive by each of BORONIC ACID and CLOXACILLIN double disc synergy tests, 35.5% were positive by the induction test, and 25.8% were plasmid-mediated AmpC β-lactamase producers by the MULTIPLEX PCR. Plasmid-mediated AMPC GENES retrieved, belonged to the families (MOX, FOX, EBC and CIT). ESBL producers were found in 26 (41.9%) isolates, 15 (57%) of which also produced AmpC. Isolates caused hospital acquired infections were (53/62); of which (39/62) were AmpC producers. While only (8/62) of the isolates caused community-acquired infections, were AmpC producers, and (1.6%) (1/62) were non AmpC producer.Conclusions: The AmpC β-lactamases detection tests had to be included in the routine microbiology workup of Gram negative bacteria, namely Cefoxitin as a screening test, combined BORONIC ACID disc test with Cefotetan, followed by synergy tests and finally by the induction test for phenotypic identifications. MULTIPLEX PCR can successfully detect the plasmid AMPC GENES.

Cites

Antibacterial Susceptibility Profiles and the Presence of Beta-Lactamase Resistance Genes and Virulence Factors in Pseudomonas aeruginosa Isolates

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APA: Copy

WASSEF, MONA, BEHIRY, IMAN, YOUNAN, MARIAM, EL GUINDY, NANCY, MOSTAFA, SALLY, & ABADA, EMAD. (2014). GENOTYPIC IDENTIFICATION OF AMPC β-LACTAMASES PRODUCTION IN GRAM-NEGATIVE BACILLI ISOLATES. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 7(1), 0-0. SID. https://sid.ir/paper/317344/en

Vancouver: Copy

WASSEF MONA, BEHIRY IMAN, YOUNAN MARIAM, EL GUINDY NANCY, MOSTAFA SALLY, ABADA EMAD. GENOTYPIC IDENTIFICATION OF AMPC β-LACTAMASES PRODUCTION IN GRAM-NEGATIVE BACILLI ISOLATES. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2014;7(1):0-0. Available from: https://sid.ir/paper/317344/en

IEEE: Copy

MONA WASSEF, IMAN BEHIRY, MARIAM YOUNAN, NANCY EL GUINDY, SALLY MOSTAFA, and EMAD ABADA, “GENOTYPIC IDENTIFICATION OF AMPC β-LACTAMASES PRODUCTION IN GRAM-NEGATIVE BACILLI ISOLATES,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 7, no. 1, pp. 0–0, 2014, [Online]. Available: https://sid.ir/paper/317344/en

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