ERADICATION OF PSEUDOMONAS AERUGINOSA BIOFILMS USING THE COMBINATION OF N-BUTANOLIC CYCLAMEN COUM EXTRACT AND CIPROFLOXACIN

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SHAFIEI MORVARID; ABDI ALI AHYA; SHAHCHERAGHI FERESHTEH; SABOORA AZRA; AKBARI NOGHABI KAMBIZ

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Journal: Jundishapur Journal of Microbiology Year:2014 | Volume:7 | Issue:2 Page(s): 0-0

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Title

ERADICATION OF PSEUDOMONAS AERUGINOSA BIOFILMS USING THE COMBINATION OF N-BUTANOLIC CYCLAMEN COUM EXTRACT AND CIPROFLOXACIN

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Keywords

PSEUDOMONAS AERUGINOSA

BIOFILM

CYCLAMEN COUM

Abstract

Background: BIOFILM formation is a major pathogenic factor in different bacteria such as PSEUDOMONAS AERUGINOSA. A number of studies have reported that bacterial BIOFILMs show different levels of antibiotic resistance. In order to re-sensitize the bacterial BIOFILMs to antibiotics, BIOFILMs should be dispersed.Objectives: In this study, the effect of n-butanolic CYCLAMEN COUM extract in combination with ciprofloxacin was examined on one, three and five day old P. aeruginosa BIOFILMs. The synergistic effect of n-butanolic C. coum extract and ciprofloxacin towards dispersing pre-established P. aeruginosa BIOFILMs was also studied.Materials and Methods: The ability of BIOFILM formation by six different P. aeruginosa strains was confirmed by microtiter plate method and PCR assay for the cupA gene. The extraction of C. coum tubers was achieved by fractionation method using different solvents. The minimum inhibitory concentration (MIC) of n-butanolic C. coum extract and ciprofloxacin against planktonic cells was evaluated using agar well diffusion and microdilution methods. The microdilution chequerboard method was used to determine the fractional BIOFILM eradication concentration index (FBCI), when the combination of n-butanolic C. coum extract and ciprofloxacin were used against P. aeruginosa BIOFILMs.Results: The ability of BIOFILM formation by P. aeruginosa strains was quantitatively confirmed. The PCR method confirmed the existence of cup A gene (172 bp) in all studied strains. Saponin content of the n-butanolic C. coum extract was 156 mg/mL. The extract revealed antibacterial activity against planktonic cells of P. aeruginosa strains. The results showed that one and three day old BIOFILMs are affected by either ciprofloxacin or n-butanolic C. coum extract. However, n-butanolic C. coum extract in combination with ciprofloxacin was significantly more effective against P. aeruginosa BIOFILMs.Conclusions: Using n-butanolic C. coum extract in combination with ciprofloxacin offers a novel strategy to control BIOFILM-based infections caused by P. aeruginosa.

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APA: Copy

SHAFIEI, MORVARID, ABDI ALI, AHYA, SHAHCHERAGHI, FERESHTEH, SABOORA, AZRA, & AKBARI NOGHABI, KAMBIZ. (2014). ERADICATION OF PSEUDOMONAS AERUGINOSA BIOFILMS USING THE COMBINATION OF N-BUTANOLIC CYCLAMEN COUM EXTRACT AND CIPROFLOXACIN. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 7(2), 0-0. SID. https://sid.ir/paper/317366/en

Vancouver: Copy

SHAFIEI MORVARID, ABDI ALI AHYA, SHAHCHERAGHI FERESHTEH, SABOORA AZRA, AKBARI NOGHABI KAMBIZ. ERADICATION OF PSEUDOMONAS AERUGINOSA BIOFILMS USING THE COMBINATION OF N-BUTANOLIC CYCLAMEN COUM EXTRACT AND CIPROFLOXACIN. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2014;7(2):0-0. Available from: https://sid.ir/paper/317366/en

IEEE: Copy

MORVARID SHAFIEI, AHYA ABDI ALI, FERESHTEH SHAHCHERAGHI, AZRA SABOORA, and KAMBIZ AKBARI NOGHABI, “ERADICATION OF PSEUDOMONAS AERUGINOSA BIOFILMS USING THE COMBINATION OF N-BUTANOLIC CYCLAMEN COUM EXTRACT AND CIPROFLOXACIN,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 7, no. 2, pp. 0–0, 2014, [Online]. Available: https://sid.ir/paper/317366/en

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