CHARACTERIZATION OF LEPTOSPIRA INTERROGANS SEROVARS BY POLYMORPHISM VARIABLE NUMBER TANDEM REPEAT ANALYSIS

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REZASOLTANI SAMA; DABIRI HOSSEIN; KHAKI PEJVAK; ROSTAMI NEJAD MOHAMMAD; KARIMNASAB NASIM; MODIRROUSTA SHIVA

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Journal: Jundishapur Journal of Microbiology Year:2015 | Volume:8 | Issue:10 Page(s): 1-6

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Title

CHARACTERIZATION OF LEPTOSPIRA INTERROGANS SEROVARS BY POLYMORPHISM VARIABLE NUMBER TANDEM REPEAT ANALYSIS

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Keywords

MLVA TECHNIQUE

VNTR ANALYSIS

POLYMORPHISM

LEPTOSPIRA SEROVARS

Abstract

Background: Leptospirosis is recognized as a re-emerging infectious disease; therefore, understanding the epidemiology of the disease is vital for designing intervention programs and diminishing its transmission. Recently, Multilocus variable number tandem repeat analysis (MLVA) is used for segregating and identifying LEPTOSPIRA SEROVARS. The method has potential application in investigating the molecular epidemiology of Leptospira.Objectives: The propose of this study was genomic identification of pathogenic Leptospires in Iran by MLVA.Materials and Methods: LEPTOSPIRA SEROVARS were obtained from National Reference Laboratory of Leptospira at Razi Vaccine and Serum Research Institute, Karaj, Iran. Serovars were cultured into the liquid EMJH medium and incubated at 28oC for 7 days. DNA of serovars was extracted using the phenol-chloroform method. PCR was performed with 5 selected variable number tandem repeat analysis (VNTR) loci. The amplified products were analyzed by agarose gel electrophoresis. The size of the amplified products was estimated by 100 bp ladder and sequencing analysis.Results: The saprophytic serovar showed no amplified fragments. PCR products in all pathogenic serovars were observed. The 12 reference serovars used for the development of technique displayed distinct patterns.Conclusions: Results showed that MLVA TECHNIQUE with its range of POLYMORPHISM is a good marker for identification of pathogenic serovars. Some VNTR loci are more powerful than the other ones with regard to differentiation. Serovars from the same geographical area have more genetic similarity than same serovars from different places. MLVA is a suitable technique for epidemiological survey.

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APA: Copy

REZASOLTANI, SAMA, DABIRI, HOSSEIN, KHAKI, PEJVAK, ROSTAMI NEJAD, MOHAMMAD, KARIMNASAB, NASIM, & MODIRROUSTA, SHIVA. (2015). CHARACTERIZATION OF LEPTOSPIRA INTERROGANS SEROVARS BY POLYMORPHISM VARIABLE NUMBER TANDEM REPEAT ANALYSIS. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 8(10), 1-6. SID. https://sid.ir/paper/317464/en

Vancouver: Copy

REZASOLTANI SAMA, DABIRI HOSSEIN, KHAKI PEJVAK, ROSTAMI NEJAD MOHAMMAD, KARIMNASAB NASIM, MODIRROUSTA SHIVA. CHARACTERIZATION OF LEPTOSPIRA INTERROGANS SEROVARS BY POLYMORPHISM VARIABLE NUMBER TANDEM REPEAT ANALYSIS. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2015;8(10):1-6. Available from: https://sid.ir/paper/317464/en

IEEE: Copy

SAMA REZASOLTANI, HOSSEIN DABIRI, PEJVAK KHAKI, MOHAMMAD ROSTAMI NEJAD, NASIM KARIMNASAB, and SHIVA MODIRROUSTA, “CHARACTERIZATION OF LEPTOSPIRA INTERROGANS SEROVARS BY POLYMORPHISM VARIABLE NUMBER TANDEM REPEAT ANALYSIS,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 8, no. 10, pp. 1–6, 2015, [Online]. Available: https://sid.ir/paper/317464/en

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