مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

CLONING AND EXPRESSION OF BETA SUBUNIT GENE OF PHYCOCYANIN FROM SPIRULINA PLATENSIS IN ESCHERICHIA COLI

Pages

  1-6

Abstract

 Background: C-Phycocyanin (C-PC) from blue-green algae such as SPIRULINA has been reported to have various pharmacological characteristics, including anti-inflammatory and anti-tumor activities. Recombinant b-subunit of C-PC (C-PC/b) is an inhibitor of cell proliferation and an inducer of cancer cell apoptosis.Objectives: Since C-PC/b has a big potential to be used as a promising cancer prevention or therapy agent, the purpose of this study was to clone and express SPIRULINA platensis cpcB gene in a bacterial expression system. This is a significant step for the production of this compound.Materials and Methods: The cpcB gene was amplified using specific primers and cloned in a bacterial expression vector, namely pET43.1a+. Gene expression of cpcB was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and the dot blotting technique.Results: The SDS-PAGE analysis and dot blotting confirmed the production of recombinant C-PC/b in the bacterial expression system. Over-expression of cpcB gene was optimized in induction by 1 mM Isopropyl-b-D-Thiogalactoside (IPTG), after four hours of inoculation at 30oC.Conclusions: Over-expression of the synthetic CPC/b protein in the bacterial system (Escherichia coli BL-21) showed that E. coli can be used as a basis for further research to produce this desired protein in large quantities.

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    Cite

    APA: Copy

    SHOJA, ZAHRA, RAJABI MEMARI, HAMID, & ROAYAEI ARDAKANI, MOHAMMD. (2015). CLONING AND EXPRESSION OF BETA SUBUNIT GENE OF PHYCOCYANIN FROM SPIRULINA PLATENSIS IN ESCHERICHIA COLI. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 8(8), 1-6. SID. https://sid.ir/paper/317505/en

    Vancouver: Copy

    SHOJA ZAHRA, RAJABI MEMARI HAMID, ROAYAEI ARDAKANI MOHAMMD. CLONING AND EXPRESSION OF BETA SUBUNIT GENE OF PHYCOCYANIN FROM SPIRULINA PLATENSIS IN ESCHERICHIA COLI. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2015;8(8):1-6. Available from: https://sid.ir/paper/317505/en

    IEEE: Copy

    ZAHRA SHOJA, HAMID RAJABI MEMARI, and MOHAMMD ROAYAEI ARDAKANI, “CLONING AND EXPRESSION OF BETA SUBUNIT GENE OF PHYCOCYANIN FROM SPIRULINA PLATENSIS IN ESCHERICHIA COLI,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 8, no. 8, pp. 1–6, 2015, [Online]. Available: https://sid.ir/paper/317505/en

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