PRODUCTION OF HUMAN ALPHA 2B INTERFERON IN E. COLI EXPRESSION SYSTEM

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ABDOLRASOULI NEHZAT; RAJABI MEMARI HAMID; ROAYAEI ARDAKANI MOHAMMAD; EBRAHIMI MOHAMAD ALI; EBRAHIMI NAZANIN

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Journal: Jundishapur Scientific Medical Journal Year:2013 | Volume:12 | Issue:3 (84) Page(s): 325-334

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Title

PRODUCTION OF HUMAN ALPHA 2B INTERFERON IN E. COLI EXPRESSION SYSTEM

Author(s)

Keywords

INTERFERON-α2BQ2

PETQ2

26B (+)Q2

ESCHERICHIA COLIQ2

IPTGQ2

Abstract

Background and Objective: Interferon’s are part of the body’s defense system against viruses. These proteins are generated in the body, and then they trigger the cells around them to produce inhibitor proteins against virus replication. The purpose of this study was cloning and expression of the human interferon alpha-2b gene in preplasmic space of Escherichia coli.Subjects and Methods: In this research, Alpha 2b interferon gene was cloned in a PET-26b (+) expression vector, under the control of T7 promoter and pelB periplasmic signal peptide sequence using NcoI and XhoI restriction enzymes. The expression vector was transformed into Escherichia coli strain BL21 (DE3). Cloning of alpha interferon gene confirmed using colony PCR, digestion and DNA sequencing. Gene expression of alpha interferon was examined by SDS-PAGE and Dot blot analysis. Also the possibility of periplasmic targeting was determined using SDS-PAGE and dot blot analysis at 15 hrs after induction.Results: The results showed that Alpha 2b interferon was produced in bacterial expression system and targeted to periplasmic space.Conclusion: Our study showed that the production of pharmaceutical recombinant protein in preplasmic space of bacterial expression system is fissible and can be utilized as a cheaper source and more efficient than conventional expression systems.

Cites

CLONING AND EXPRESSION OF BETA SUBUNIT GENE OF PHYCOCYANIN FROM SPIRULINA PLATENSIS IN ESCHERICHIA COLI

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APA: Copy

ABDOLRASOULI, NEHZAT, RAJABI MEMARI, HAMID, ROAYAEI ARDAKANI, MOHAMMAD, EBRAHIMI, MOHAMAD ALI, & EBRAHIMI, NAZANIN. (2013). PRODUCTION OF HUMAN ALPHA 2B INTERFERON IN E. COLI EXPRESSION SYSTEM. JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL, 12(3 (84)), 325-334. SID. https://sid.ir/paper/12759/en

Vancouver: Copy

ABDOLRASOULI NEHZAT, RAJABI MEMARI HAMID, ROAYAEI ARDAKANI MOHAMMAD, EBRAHIMI MOHAMAD ALI, EBRAHIMI NAZANIN. PRODUCTION OF HUMAN ALPHA 2B INTERFERON IN E. COLI EXPRESSION SYSTEM. JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL[Internet]. 2013;12(3 (84)):325-334. Available from: https://sid.ir/paper/12759/en

IEEE: Copy

NEHZAT ABDOLRASOULI, HAMID RAJABI MEMARI, MOHAMMAD ROAYAEI ARDAKANI, MOHAMAD ALI EBRAHIMI, and NAZANIN EBRAHIMI, “PRODUCTION OF HUMAN ALPHA 2B INTERFERON IN E. COLI EXPRESSION SYSTEM,” JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL, vol. 12, no. 3 (84), pp. 325–334, 2013, [Online]. Available: https://sid.ir/paper/12759/en

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