ANTI-METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS ACTIVITY AND OPTIMAL CULTURE CONDITION OF STREPTOMYCES SP. SUK 25

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SITI JUNAIDAH AHMAD; SUHAINI SUDI; MOHD SIDEK HASIDAH; BASRI DAYANG FREDALINA; ZIN NORAZIAH MOHAMAD

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Journal: Jundishapur Journal of Microbiology Year:2015 | Volume:8 | Issue:5 Page(s): 0-0

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Title

ANTI-METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS ACTIVITY AND OPTIMAL CULTURE CONDITION OF STREPTOMYCES SP. SUK 25

Author(s)

Keywords

STREPTOMYCES SP.

MRSA

CULTURE

Abstract

Background: The potential of secondary metabolites extracted from STREPTOMYCES SP. to treat bacterial infections including infections with Staphylococcus aureus is previously documented. The current study showed significant antimicrobial activities associated with endophytic STREPTOMYCES SP. isolated from medicinal plants in Peninsular Malaysia.Objectives: The current study aimed to determine anti-methicillin-resistant-Staphylococcus aureus (MRSA) activities of STREPTOMYCES SP. isolates.Materials and Methods: Disc diffusion and Minimum Inhibitory Concentration (MIC) assay were used to determine the antibacterial activity of STREPTOMYCES SP. isolates. Optimization of fermentation parameters for the most potent anti-MRSA extract in terms of medium type, pH, aeration rate, and CULTURE period was also carried out. Lastly, toxicity of the extract against Chang liver cells was determined employing the MTT, 2- (3, 5- diphenyltetrazol-2-ium-2-yl) -4, 5-dimethyl -1, 3 - thiazole; bromide assay.Results: The results indicated STREPTOMYCES SP. SUK 25 isolates showed the most potent anti-MRSA activity. Disc diffusion assay revealed that spread plate technique was more efficient in screening anti-MRSA activity compared to pour plate (P<0.05). To determine anti–MRSA MIC of STREPTOMYCES SP. SUK 25, Thronton media was used. Therefore, MIC was determined as 2.44±0.01 mg/mL, and accordingly, the lowest MIC was 1.95 mg/mL based on a seven-day CULTURE, pH7, and aeration rate of 140 rpm. The crude extract was not toxic against Chang liver cells (IC50=43.31±1.24 mg/mL).Conclusions: The STREPTOMYCES SP. SUK 25 culturing was optimized using Thronton media, at pH 7 and aeration of 140 rpm. Further isolation and identification of bioactive compounds will develop anti-MRSA therapeutics.

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APA: Copy

SITI JUNAIDAH, AHMAD, SUHAINI, SUDI, MOHD SIDEK, HASIDAH, BASRI, DAYANG FREDALINA, & ZIN, NORAZIAH MOHAMAD. (2015). ANTI-METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS ACTIVITY AND OPTIMAL CULTURE CONDITION OF STREPTOMYCES SP. SUK 25. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 8(5), 0-0. SID. https://sid.ir/paper/317546/en

Vancouver: Copy

SITI JUNAIDAH AHMAD, SUHAINI SUDI, MOHD SIDEK HASIDAH, BASRI DAYANG FREDALINA, ZIN NORAZIAH MOHAMAD. ANTI-METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS ACTIVITY AND OPTIMAL CULTURE CONDITION OF STREPTOMYCES SP. SUK 25. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2015;8(5):0-0. Available from: https://sid.ir/paper/317546/en

IEEE: Copy

AHMAD SITI JUNAIDAH, SUDI SUHAINI, HASIDAH MOHD SIDEK, DAYANG FREDALINA BASRI, and NORAZIAH MOHAMAD ZIN, “ANTI-METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS ACTIVITY AND OPTIMAL CULTURE CONDITION OF STREPTOMYCES SP. SUK 25,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 8, no. 5, pp. 0–0, 2015, [Online]. Available: https://sid.ir/paper/317546/en

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