A Molecular Approach to Nested RT-PCR Using a New Set of Primers for the Detection of the Human Immunodeficiency Virus Protease Gene

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ZAREI MOHAMMAD; RAVANSHAD MEHRDAD; Bagban Ashraf; Fallahi Shahab

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Journal Paper

Paper Information

Journal: Jundishapur Journal of Microbiology Year:2016 | Volume:9 | Issue:7 Page(s): 0-0

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Title

A Molecular Approach to Nested RT-PCR Using a New Set of Primers for the Detection of the Human Immunodeficiency Virus Protease Gene

Author(s)

ZAREI MOHAMMAD | RAVANSHAD MEHRDAD | Bagban Ashraf | Fallahi Shahab | Issue Writer Certificate

Keywords

HIV-1

Nested PCR

HIV Protease

Abstract

Background: The human immunodeficiency virus (HIV-1) is the etiologic agent of AIDS. The disease can be transmitted via blood in the window period prior to the development of antibodies to the disease. Thus, an appropriate method for the detection of HIV-1 during this window period is very important. Objectives: This descriptive study proposes a sensitive, efficient, inexpensive, and easy method to detect HIV-1. Patients and Methods: In this study 25 serum samples of patients under treatment and also 10 positive and 10 negative control samples were studied. Twenty-five blood samples were obtained from HIV-1-infected individuals who were receiving treatment at the acquired immune deficiency syndrome (AIDS) research center of Imam Khomeini hospital in Tehran. The identification of HIV-1-positive samples was done by using reverse transcription to produce copy deoxyribonucleic acid (cDNA) and then optimizing the nested polymerase chain reaction (PCR) method. Two pairs of primers were then designed specifically for the protease gene fragmentof the nested real time-PCR (RT-PCR) samples. Electrophoresiswasused to examinethePCRproducts. The results were analyzed using statistical tests, including Fisher’ s exact test, and SPSS17 software. Results: The 325 bp band of the protease gene was observed in all the positive control samples and in none of the negative control samples. The proposed method correctly identified HIV-1 in 23 of the 25 samples. Conclusions: These results suggest that, in comparison with viral cultures, antibody detection by enzyme linked immunosorbent assay (ELISAs), and conventional PCR methods, the proposed method has high sensitivity and specificity for the detection of HIV-1.

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APA: Copy

ZAREI, MOHAMMAD, RAVANSHAD, MEHRDAD, Bagban, Ashraf, & Fallahi, Shahab. (2016). A Molecular Approach to Nested RT-PCR Using a New Set of Primers for the Detection of the Human Immunodeficiency Virus Protease Gene. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 9(7), 0-0. SID. https://sid.ir/paper/317762/en

Vancouver: Copy

ZAREI MOHAMMAD, RAVANSHAD MEHRDAD, Bagban Ashraf, Fallahi Shahab. A Molecular Approach to Nested RT-PCR Using a New Set of Primers for the Detection of the Human Immunodeficiency Virus Protease Gene. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2016;9(7):0-0. Available from: https://sid.ir/paper/317762/en

IEEE: Copy

MOHAMMAD ZAREI, MEHRDAD RAVANSHAD, Ashraf Bagban, and Shahab Fallahi, “A Molecular Approach to Nested RT-PCR Using a New Set of Primers for the Detection of the Human Immunodeficiency Virus Protease Gene,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 9, no. 7, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/317762/en

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