Rapid Detection of Rifampicin-and Isoniazid-Resistant Mycobacterium tuberculosis Using Real-Time PCR

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SAHEBI LEYLA; ANSARIN KHALIL; MONFAREDAN AMIR; FARAJNIA SAFAR; Nili Seiran; KHALILI MAJID

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Jundishapur Journal of Microbiology Year:2016 | Volume:9 | Issue:10 Page(s): 0-0

Download Full-Text

View:

190

Download:

105

Cites:

Information Journal Paper

Title

Rapid Detection of Rifampicin-and Isoniazid-Resistant Mycobacterium tuberculosis Using Real-Time PCR

Author(s)

Keywords

Drug Resistance

Real-Time PCR

Melt Curve Technique

Mycobacterium tuberculosis

Abstract

Background: Accurateandrapid detection of drug-resistant Mycobacterium tuberculosis is fundamental for the successful treatment of tuberculosis (TB). Objectives: The aim of this study was to determine the frequency of common mutations leading to isoniazid (INH) and rifampicin (RMP) resistance. Patients and Methods: In a cross-sectional study carried out in 2014, 90 patients with M. tuberculosis from five border provinces of Iran were selected. After a full clinical history and physical evaluation, real-time polymerase chain reaction (PCR) technique was performed for the detection of mutations in the patients’ katG and rpoB genes. The results were compared with results of a standard proportion method as well as a multiplex allele-specific PCR (MAS-PCR). Results: A total of 23 mutations were found in isolates among which, codon katG 315, rpoB P1 (511-519 sequence) and rpoB P2 (524-533 sequence) were responsible for seven, nineandseven cases, respectively. Themean(standard deviation (SD)) of melting temperature (Tm) in katG 315 codon, rpoB P1 and P2 sequences in susceptible and mutant isolates was as follows: katG 85. 4° C (0. 18) and 87. 54° C (0. 62); rpoB P1 84. 6° C (0. 61) and 82. 9° C (0. 38); rpoB P2 83. 4° C (0. 18) and 85. 3° C (0. 19), respectively. In comparison to the standard proportion test, the sensitivity of Real-Time PCR in detecting INH-and RMP-resistant mutations was 75% and 83. 3%, respectively. In comparison to theMAS-PCRtest, 100% of katG 315 mutationsand80% of rpoB mutations were determined. Overall, 10% of the patients were diagnosed with a recurrence of TB. Age and previous history of TB treatment increased mutation odds in rpoB sequences (P = 0. 046, P = 0. 036, respectively). Conclusions: Detection of Drug Resistance associated with mutations through Real-Time PCR by melting analysis technique showed a high differentiating power. This technique had high concordance with the standard proportion test and MAS-PCR results.

Cites

No record.

References

No record.

Cite

APA: Copy

SAHEBI, LEYLA, ANSARIN, KHALIL, MONFAREDAN, AMIR, FARAJNIA, SAFAR, Nili, Seiran, & KHALILI, MAJID. (2016). Rapid Detection of Rifampicin-and Isoniazid-Resistant Mycobacterium tuberculosis Using Real-Time PCR. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 9(10), 0-0. SID. https://sid.ir/paper/317769/en

Vancouver: Copy

SAHEBI LEYLA, ANSARIN KHALIL, MONFAREDAN AMIR, FARAJNIA SAFAR, Nili Seiran, KHALILI MAJID. Rapid Detection of Rifampicin-and Isoniazid-Resistant Mycobacterium tuberculosis Using Real-Time PCR. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2016;9(10):0-0. Available from: https://sid.ir/paper/317769/en

IEEE: Copy

LEYLA SAHEBI, KHALIL ANSARIN, AMIR MONFAREDAN, SAFAR FARAJNIA, Seiran Nili, and MAJID KHALILI, “Rapid Detection of Rifampicin-and Isoniazid-Resistant Mycobacterium tuberculosis Using Real-Time PCR,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 9, no. 10, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/317769/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops