Isolation, Characterization, and Antibacterial Activity of Bacteriophages Against Methicillin-Resistant Staphylococcus aureus in Pakistan

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Rasool Muhammad Hidayat; Yousaf Rukhsana; Siddique Abu Baker; Saqalein Muhammad; KHURSHID MOHSIN

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Journal: Jundishapur Journal of Microbiology Year:2016 | Volume:9 | Issue:10 Page(s): 0-0

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Title

Isolation, Characterization, and Antibacterial Activity of Bacteriophages Against Methicillin-Resistant Staphylococcus aureus in Pakistan

Author(s)

Keywords

MRSA

Bacteriophage

SDS-PAGE

Clear Plaques

In-Vitro Efficacy

In-Vivo Model

Abstract

Background: In recent years, antibiotic resistance has been indicated as a paramount threat to public health. The use of Bacteriophages appears to be a safer alternative for the control of bacterial infections. Objectives: The present study aims to explore sewage water for the presence of indigenous Bacteriophages, and to investigate their antibacterial potential against Methicillin-resistant Staphylococcus aureus (MRSA). Methods: Bacterial isolates were first collected and identified from pus samples taken from the surgical and burn units using standard microbiological procedures. A cefoxitin disk screen test was then used and interpreted according to the clinical laboratory standards institute (CLSI) guidelines for the detection of MRSA. The sewage samples were processed and the phages enriched using S. aureus as a host organism. Turbid and Clear Plaques of different sizes were isolated using an overlay method, purified, and then enumerated by means of a dilution method. Results: The phages exhibited good lytic activity against MRSA when tested in-vitro, and the highest activity was attained within three to six hours of phage infection. The isolated phage pq/48 was also found efficient in decreasing the bacterial count during an in-vivo trial in rabbits. A protein analysis using SDS-PAGE revealed 10 proteins of between 20 kDa and 155 kDa in size. Conclusions: The overall results indicated that Bacteriophages isolated from sewage exhibited excellent lytic activity against MRSA strains. In conclusion, Bacteriophages can be further characterized and appear to be a promising candidate for phage therapy against MRSA in the future.

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APA: Copy

Rasool, Muhammad Hidayat, Yousaf, Rukhsana, Siddique, Abu Baker, Saqalein, Muhammad, & KHURSHID, MOHSIN. (2016). Isolation, Characterization, and Antibacterial Activity of Bacteriophages Against Methicillin-Resistant Staphylococcus aureus in Pakistan. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 9(10), 0-0. SID. https://sid.ir/paper/317779/en

Vancouver: Copy

Rasool Muhammad Hidayat, Yousaf Rukhsana, Siddique Abu Baker, Saqalein Muhammad, KHURSHID MOHSIN. Isolation, Characterization, and Antibacterial Activity of Bacteriophages Against Methicillin-Resistant Staphylococcus aureus in Pakistan. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2016;9(10):0-0. Available from: https://sid.ir/paper/317779/en

IEEE: Copy

Muhammad Hidayat Rasool, Rukhsana Yousaf, Abu Baker Siddique, Muhammad Saqalein, and MOHSIN KHURSHID, “Isolation, Characterization, and Antibacterial Activity of Bacteriophages Against Methicillin-Resistant Staphylococcus aureus in Pakistan,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 9, no. 10, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/317779/en

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