FLASH-PCR as a Simple and Efficient Method for Detection of Brucella spp. Infection

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MORADABADI ALIREZA; AHMADI AZAM; KAHBAZI MANIJEH; SARMADIAN HOSSEIN; ARJOMANDZADEGAN MOHAMMAD

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Journal Paper

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Journal: Jundishapur Journal of Microbiology Year:2019 | Volume:12 | Issue:4 Page(s): 0-0

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Title

FLASH-PCR as a Simple and Efficient Method for Detection of Brucella spp. Infection

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Keywords

Brucellosis

Brucella

FLASH-PCR

Abstract

Background: Brucella spp. are Gram-positive, rod-shaped, and spore-forming bacilli. Brucella abortus and B. melitensis are the main causes of Brucellosis. Objectives: The aim of the study was to establish a rapid and simple molecular method for the detection of this disease. Methods: Forty-five Brucella spp. were isolated from blood samples using the BACTEC Fluorescent 9050 system and were detected by anti-IgM or IgG Brucella specific antigen. DNA extraction was conducted on all samples. Fluorescent amplification based specific hybridization (FLASH-PCR) test was utilized to detect the 351-bp fragment from eryD gene, which was specific for Brucella spp. Results: A351-bp fragment resulted fromPCRreactionandshowedthe accuracy of designed primers. This fragmentwassuccessfully amplified in the FLASH-PCR reaction. In this study, we have positive and negative samples and a standard method. In addition, we calculated the sensitivity and specificity of this method as 100%. Conclusions: Results of the study was proved that the FLASH-PCR method was a rapid, sensitive, and safe method for the detection of Brucella genome in whole blood samples of patient harbored Brucellosis and is recommended for routine usage.

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APA: Copy

MORADABADI, ALIREZA, AHMADI, AZAM, KAHBAZI, MANIJEH, SARMADIAN, HOSSEIN, & ARJOMANDZADEGAN, MOHAMMAD. (2019). FLASH-PCR as a Simple and Efficient Method for Detection of Brucella spp. Infection. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 12(4), 0-0. SID. https://sid.ir/paper/317863/en

Vancouver: Copy

MORADABADI ALIREZA, AHMADI AZAM, KAHBAZI MANIJEH, SARMADIAN HOSSEIN, ARJOMANDZADEGAN MOHAMMAD. FLASH-PCR as a Simple and Efficient Method for Detection of Brucella spp. Infection. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2019;12(4):0-0. Available from: https://sid.ir/paper/317863/en

IEEE: Copy

ALIREZA MORADABADI, AZAM AHMADI, MANIJEH KAHBAZI, HOSSEIN SARMADIAN, and MOHAMMAD ARJOMANDZADEGAN, “FLASH-PCR as a Simple and Efficient Method for Detection of Brucella spp. Infection,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 12, no. 4, pp. 0–0, 2019, [Online]. Available: https://sid.ir/paper/317863/en

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