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Information Journal Paper

Title

A New Medium for Improving Spinosad Production by Saccharopolyspora spinosa

Pages

  0-0

Abstract

 Background: Spinosad (a mixture of Spinosyns A and D) is a unique natural pesticide produced by Saccharopolyspora spinosa. With regard to attempts to improve S. spinosa by classical mutagenesis, we propose that the bottleneck of screening out high-spinosadproduction strains is probably caused by the fermentation media. Objectives: The current study aimed to identify a new medium to extensively investigate the potential of S. spinosa strains to produce spinosad. Materials and Methods: Statistical and regressive modeling methods were used to investigate the effects of the carbon source and to optimize the production media. Results: The spinosad production of S. spinosa Co121 increased 77. 13%, from 310. 44 21. 84 g/mL in the initial fermentation medium (with glucose as the main carbon source) to 549. 89  38. 59  g/mL in a new optimized fermentation medium (98. 0 g of Mannitol, 43. 0 g of cottonseed flour, 12. 9 g of corn steep liquor, 0. 5 g of KH2PO4, and 3. 0 g of CaCO3 in 1 L of H2O; pH was adjusted to 7. 0 before autoclaving). After screening 4, 000 strains, an overall 3. 33-fold increase was observed in spinosad titers, starting from the parental strain Co121 in the original fermentation medium and ending with the mutant strain J78 (1035  34  g/mL) in the optimized medium. Conclusions: The optimized fermentation medium developed in this study can probably be used to improve spinosad production in screening industrial strains of S. spinosa.

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    APA: Copy

    Guojun, Yang, Yuping, he, Yan, Jiang, & Kaichun, Lin. (2016). A New Medium for Improving Spinosad Production by Saccharopolyspora spinosa. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 9(6), 0-0. SID. https://sid.ir/paper/317890/en

    Vancouver: Copy

    Guojun Yang, Yuping he, Yan Jiang, Kaichun Lin. A New Medium for Improving Spinosad Production by Saccharopolyspora spinosa. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2016;9(6):0-0. Available from: https://sid.ir/paper/317890/en

    IEEE: Copy

    Yang Guojun, he Yuping, Jiang Yan, and Lin Kaichun, “A New Medium for Improving Spinosad Production by Saccharopolyspora spinosa,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 9, no. 6, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/317890/en

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