Antimicrobial Resistance, Biofilm Formation, and Phylogenetic Grouping of Uropathogenic Escherichia coli Isolates in Egypt: The Role of Efflux Pump-Mediated Resistance

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Gawad Wesam Elsayed; Helmy Omneya Mohamed; Tawakkol Wael Mostafa; Hashem Abdelgawad Mohamed

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Journal Paper

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Journal: Jundishapur Journal of Microbiology Year:2018 | Volume:11 | Issue:2 Page(s): 0-0

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Title

Antimicrobial Resistance, Biofilm Formation, and Phylogenetic Grouping of Uropathogenic Escherichia coli Isolates in Egypt: The Role of Efflux Pump-Mediated Resistance

Author(s)

Gawad Wesam Elsayed | Helmy Omneya Mohamed | Tawakkol Wael Mostafa | Hashem Abdelgawad Mohamed | Issue Writer Certificate

Keywords

Biofilms

Drug Resistance

Uropathogenic Escherichia coli

Abstract

Background: Urinary tract infections are the most commonly encountered infections in clinics and outpatient settings and are mainly caused by Uropathogenic Escherichia coli (UPEC). Multidrug-resistant bacteria (MDR) have become a major public health threat, worldwide. Objectives: This study aimed to investigate the prevalence of theMDRphenotype, efflux pump-mediated resistance, and the ability for in vitro biofilm formation among UPEC clinical isolates from Egypt. Methods: Uropathogenic E. coli isolates were collected from two Egyptian governorates, identified, and classified to their corresponding phylogenetic group by the polymerase chain reaction (PCR). Antimicrobial susceptibility testing was done using the Kirby-Bauer disk diffusion method. AcrAB-TolC efflux pump major genes were detected by PCR; efflux pump-mediated resistance was determined by the efflux pump inhibitor microplate-based assay. The ability for in-vitro biofilm formation was also tested. Results: The phylogenetic analysis of the UPEC isolates revealed that most of the isolates belonged to groups B2 and D. The MDR phenotype was detected in 90. 8% of UPEC isolates; efflux pump-mediated resistance was detected in all MDR isolates. The acrA, acrB, and tolC were detected in 74. 84% of MDRisolates. The ability for in-vitro biofilm formation was recorded in 76. 5% of theUPECisolates. Conclusions: The MDR phenotype and the ability for in-vitro biofilm formation were predominant among UPEC in Egypt. The high prevalence of MDR efflux pumps necessitates the application of new treatment strategies to inhibit this phenomenon.

Cites

Evaluation of Biofilm Formation and Frequency of Genes Encoding Curli Fiber, Colanic Acid Capsule and F1c Fimberia Among Uropathogenic Escherichia coli Isolates With Strong Cell Surface Hydrophobicity

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APA: Copy

Gawad, Wesam Elsayed, Helmy, Omneya Mohamed, Tawakkol, Wael Mostafa, & Hashem, Abdelgawad Mohamed. (2018). Antimicrobial Resistance, Biofilm Formation, and Phylogenetic Grouping of Uropathogenic Escherichia coli Isolates in Egypt: The Role of Efflux Pump-Mediated Resistance. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 11(2), 0-0. SID. https://sid.ir/paper/317942/en

Vancouver: Copy

Gawad Wesam Elsayed, Helmy Omneya Mohamed, Tawakkol Wael Mostafa, Hashem Abdelgawad Mohamed. Antimicrobial Resistance, Biofilm Formation, and Phylogenetic Grouping of Uropathogenic Escherichia coli Isolates in Egypt: The Role of Efflux Pump-Mediated Resistance. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2018;11(2):0-0. Available from: https://sid.ir/paper/317942/en

IEEE: Copy

Wesam Elsayed Gawad, Omneya Mohamed Helmy, Wael Mostafa Tawakkol, and Abdelgawad Mohamed Hashem, “Antimicrobial Resistance, Biofilm Formation, and Phylogenetic Grouping of Uropathogenic Escherichia coli Isolates in Egypt: The Role of Efflux Pump-Mediated Resistance,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 11, no. 2, pp. 0–0, 2018, [Online]. Available: https://sid.ir/paper/317942/en

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