High Prevalence of tetMas Compared to tetK Amongst Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Hospitals in Perak, Malaysia

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Ong Melissa Hui Ling; Ho Wai Yew; Ng Wei Wen; Chew Choy Hoong

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Journal: Jundishapur Journal of Microbiology Year:2017 | Volume:10 | Issue:6 Page(s): 0-0

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Title

High Prevalence of tetMas Compared to tetK Amongst Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Hospitals in Perak, Malaysia

Author(s)

Ong Melissa Hui Ling | Ho Wai Yew | Ng Wei Wen | Chew Choy Hoong | Issue Writer Certificate

Keywords

tetM

tetK

Sccmec

Tetracycline Resistance

MRSA

Duplex PCR

Abstract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) has become a great concern to public health as it is one of the most successful and adaptable human pathogens. Antibiotic is still the main treatment for infected patients. Therefore, identifying the prevalence of antibiotic resistant genes is essential to reduce the mortality and morbility rate of MRSA-infected patients. Objectives: This study aimed to identify the prevalence of Tetracycline Resistance of MRSA and its determinants (tetK and tetM) and their relationship with Sccmec types. Methods: One hundred and seventeen MRSA isolates were collected from different body sites (eg, blood, pus, tissue, synovial fluid, eye, spinal fluid, wound, and nasal cavity) from patients with age of 1-to 90-years old. Antibiotic susceptibility tests were carried out in order to determine tetracycline resistant MRSA. Total DNA was extracted using modified spin column method and subjected to Duplex PCR for the amplification of genes of interests (mecA, tetK and tetM) and multiplex PCR for the Sccmec types identification. Results: Out of 117 MRSA isolates, 76. 1% were found to be tetracycline resistant, 8. 5% were intermediate resistant and 15. 4% were susceptible to tetracycline. Among the tetracycline resistant isolates, 97. 8% carried tetM, while 42. 7% carried tetK. The two genetic determinants were found mostly associated with Sccmec type III MRSA, whereby 95. 0% harbored tetM while 37. 0% co-carried tetK. tetK was presented alone (9. 1%) in Sccmec type IV MRSA, although tetMwas found in Sccmec type V MRSA. Conclusions: Tetracycline determinant tetMis more prevalent than tetK in this region of study and most of these determinants are found encoded in Sccmec type III. Since tetracycline antibiotic is losing its efficacy, this antibiotic should be prescribed more wisely.

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APA: Copy

Ong, Melissa Hui Ling, Ho, Wai Yew, Ng, Wei Wen, & Chew, Choy Hoong. (2017). High Prevalence of tetMas Compared to tetK Amongst Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Hospitals in Perak, Malaysia. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 10(6), 0-0. SID. https://sid.ir/paper/317961/en

Vancouver: Copy

Ong Melissa Hui Ling, Ho Wai Yew, Ng Wei Wen, Chew Choy Hoong. High Prevalence of tetMas Compared to tetK Amongst Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Hospitals in Perak, Malaysia. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2017;10(6):0-0. Available from: https://sid.ir/paper/317961/en

IEEE: Copy

Melissa Hui Ling Ong, Wai Yew Ho, Wei Wen Ng, and Choy Hoong Chew, “High Prevalence of tetMas Compared to tetK Amongst Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Hospitals in Perak, Malaysia,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 10, no. 6, pp. 0–0, 2017, [Online]. Available: https://sid.ir/paper/317961/en

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