OPTIMIZATION OF ALKALINE PHOSPHATASE GENE EXPRESSION IN E. COLI

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MIRMOHAMMAD SADEGHI HAMID; RABANI M.; FAZELI S.

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Journal Paper

Paper Information

Journal: RESEARCH IN PHARMACEUTICAL SCIENCES (RPS) Year:2008 | Volume:3 | Issue:1 Page(s): 35-39

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Title

OPTIMIZATION OF ALKALINE PHOSPHATASE GENE EXPRESSION IN E. COLI

Author(s)

MIRMOHAMMAD SADEGHI HAMID | RABANI M. | FAZELI S. | Issue Writer Certificate

Keywords

ALKALINE PHOSPHATASE

PET15B

E. COLI K-12

Abstract

ALKALINE PHOSPHATASE is an enzyme with widespread use in research and industry such as protein labeling, dephosphorylation of nucleic acids, and enzyme based biosensors. In the present study, ALKALINE PHOSPHATASE gene was inserted into the PET15B vector. The recombinant DNA was then expressed using IPTG as an inducer. The expression of this enzyme was optimized by changing expression conditions such as IPTG concentration, time, and temperature. The obtained results demonstrate that with the expression system used, large quantities of active ALKALINE PHOSPHATASE can be produced in E. coli.

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APA: Copy

MIRMOHAMMAD SADEGHI, HAMID, RABANI, M., & FAZELI, S.. (2008). OPTIMIZATION OF ALKALINE PHOSPHATASE GENE EXPRESSION IN E. COLI. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), 3(1), 35-39. SID. https://sid.ir/paper/318261/en

Vancouver: Copy

MIRMOHAMMAD SADEGHI HAMID, RABANI M., FAZELI S.. OPTIMIZATION OF ALKALINE PHOSPHATASE GENE EXPRESSION IN E. COLI. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS)[Internet]. 2008;3(1):35-39. Available from: https://sid.ir/paper/318261/en

IEEE: Copy

HAMID MIRMOHAMMAD SADEGHI, M. RABANI, and S. FAZELI, “OPTIMIZATION OF ALKALINE PHOSPHATASE GENE EXPRESSION IN E. COLI,” RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), vol. 3, no. 1, pp. 35–39, 2008, [Online]. Available: https://sid.ir/paper/318261/en

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