QUANTITATIVE EVALUATION OF DNMT3B PROMOTER METHYLATION IN BREAST CANCER PATIENTS USING DIFFERENTIAL HIGH RESOLUTION MELTING ANALYSIS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NAGHITORABI M.; MOHAMMADI ASL J.; MIR MOHAMMAD SADEGHI H.; RABBANI M.; JAFARIAN DEHKORDI A.; HAGHJOOYE JAVANMARD S.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: RESEARCH IN PHARMACEUTICAL SCIENCES (RPS) Year:2013 | Volume:8 | Issue:3 Page(s): 167-175

Download Full-Text

View:

348

Download:

120

Cites:

Information Journal Paper

Title

QUANTITATIVE EVALUATION OF DNMT3B PROMOTER METHYLATION IN BREAST CANCER PATIENTS USING DIFFERENTIAL HIGH RESOLUTION MELTING ANALYSIS

Author(s)

Keywords

DNA METHYLATION

DNMT3B

BREAST CANCER

HIGH RESOLUTION MELTING ANALYSIS

FFPE

Abstract

DNA METHYLATION plays an important role in carcinogenesis through epigenetic silencing of tumor suppressor genes. Aberrant methylation usually results from changes in the activity of DNA methyltransferases (DNMTs). Some studies show that the overexpression of the DNMTs may lead to aberrant methylation of tumor suppressor genes. Also the overexpression of DNMTs may be related to methylation status of their genes. Due to limited number of studies on DNMT3B promoter methylation, this study was performed to quantitatively measure the methylation level of DNMT3B gene in archival formalin fixed paraffin embedded (FFPE) tissues from BREAST CANCER patients. Using differential HIGH RESOLUTION MELTING ANALYSIS (D-HRMA) technology, the methylation level of DNMT3B gene promoter was quantified in 98 BREAST CANCER FFPE tissues and also 10 fresh frozen normal tissue samples. Statistical analyses used for analyzing the correlation between the methylation and clinical variables. All the normal samples were found to be methylated at the DNMT3B promoter (the average methylation level 3.34%). Patients were identified as hypo-methylated (mean methylation level 0.8%), methylated (mean methylation level 2.48%) and hyper-methylated (mean methylation level 10.5%). Statistical analysis showed a significant correlation between the methylation status and the sample type, cancer type and tumor size. Also the methylation level was significantly associated with histologic grade. It is concluded that quantification of DNMT3B promoter methylation might be used as a reliable and sensitive diagnostic and prognostic tool in BREAST CANCER. Also D-HRMA is demonstrated as a rapid and cost effective method for quantitative evaluation of promoter methylation.

Multimedia

No record.

Cites

No record.

References

No record.

Cite

APA: Copy

NAGHITORABI, M., MOHAMMADI ASL, J., MIR MOHAMMAD SADEGHI, H., RABBANI, M., JAFARIAN DEHKORDI, A., & HAGHJOOYE JAVANMARD, S.. (2013). QUANTITATIVE EVALUATION OF DNMT3B PROMOTER METHYLATION IN BREAST CANCER PATIENTS USING DIFFERENTIAL HIGH RESOLUTION MELTING ANALYSIS. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), 8(3), 167-175. SID. https://sid.ir/paper/318348/en

Vancouver: Copy

NAGHITORABI M., MOHAMMADI ASL J., MIR MOHAMMAD SADEGHI H., RABBANI M., JAFARIAN DEHKORDI A., HAGHJOOYE JAVANMARD S.. QUANTITATIVE EVALUATION OF DNMT3B PROMOTER METHYLATION IN BREAST CANCER PATIENTS USING DIFFERENTIAL HIGH RESOLUTION MELTING ANALYSIS. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS)[Internet]. 2013;8(3):167-175. Available from: https://sid.ir/paper/318348/en

IEEE: Copy

M. NAGHITORABI, J. MOHAMMADI ASL, H. MIR MOHAMMAD SADEGHI, M. RABBANI, A. JAFARIAN DEHKORDI, and S. HAGHJOOYE JAVANMARD, “QUANTITATIVE EVALUATION OF DNMT3B PROMOTER METHYLATION IN BREAST CANCER PATIENTS USING DIFFERENTIAL HIGH RESOLUTION MELTING ANALYSIS,” RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), vol. 8, no. 3, pp. 167–175, 2013, [Online]. Available: https://sid.ir/paper/318348/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops