مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

383
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

168
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

2

Information Journal Paper

Title

THE APOPTOTIC EFFECTS OF SIRTUIN1 INHIBITOR ON THE MCF-7 AND MRC-5 CELL LINES

Pages

  79-89

Abstract

 Sirtuin1 (SIRT1) is an enzyme that deacetylates histones and several nonhistone proteins including p53 during stress and plays an important role in the survival of tumor cells. Hereby, this study describes the potency of SALERMIDE as a SIRT1 inhibitor to induce APOPTOSIS in the MCF-7 and MRC-5 cell lines. MCF7 and MRC-5 cell lines were cultured in RPMI-1640 and treated with or without SALERMIDE at concentration of 80.56 mmol/L, based on the half-maximal inhibitory concentration (IC50) index at different times (24, 48 and72 h). The IC50 value was established for the SALERMIDE in MCF-7. The percentage of apoptotic cells was measured by flow cytometry. Real-time quantitative RT-PCR was performed to estimate the mRNA expression of SIRTUIN1 in MCF-7 and MRC-5 with SALERMIDE at different times. ELISA and Bradford protein techniques were used to detect endogenous levels of total and acetylated p53 protein generated in MCF-7 and MRC-5 cells. Our findings indicated that SALERMIDE can induce APOPTOSIS in MCF-7 significantly more effective than MRC-5 cells. We showed that the expression of SIRT1 was dramatically down-regulated by increasing the time of SALERMIDE treatment in MCF-7 but not MRC-5 and that the acetylated and total p53 protein levels were increased more in MCF-7 than MRC-5. SALERMIDE, by decreasing the expression of SIRTUIN1 gene, can induce acetylation of P53 protein and consequently induce significant cell death in MCF-7 that was well tolerated in MRC-5.

Cites

References

  • No record.

    • Cite

    APA: Copy

    NIKBAKHT DASTJERDI, M., SALAHSHOOR, M.R., MARDANI, M., RABBANI, M., HASHEMIBENI, B., GHARAGOZLOO, M., KAZEMI, M., ESMAEIL, N., ROSHANKHAH, SH., GOLMOHAMMADI, R., & MOBARAKIAN, M.. (2013). THE APOPTOTIC EFFECTS OF SIRTUIN1 INHIBITOR ON THE MCF-7 AND MRC-5 CELL LINES. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), 8(2), 79-89. SID. https://sid.ir/paper/318356/en

    Vancouver: Copy

    NIKBAKHT DASTJERDI M., SALAHSHOOR M.R., MARDANI M., RABBANI M., HASHEMIBENI B., GHARAGOZLOO M., KAZEMI M., ESMAEIL N., ROSHANKHAH SH., GOLMOHAMMADI R., MOBARAKIAN M.. THE APOPTOTIC EFFECTS OF SIRTUIN1 INHIBITOR ON THE MCF-7 AND MRC-5 CELL LINES. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS)[Internet]. 2013;8(2):79-89. Available from: https://sid.ir/paper/318356/en

    IEEE: Copy

    M. NIKBAKHT DASTJERDI, M.R. SALAHSHOOR, M. MARDANI, M. RABBANI, B. HASHEMIBENI, M. GHARAGOZLOO, M. KAZEMI, N. ESMAEIL, SH. ROSHANKHAH, R. GOLMOHAMMADI, and M. MOBARAKIAN, “THE APOPTOTIC EFFECTS OF SIRTUIN1 INHIBITOR ON THE MCF-7 AND MRC-5 CELL LINES,” RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), vol. 8, no. 2, pp. 79–89, 2013, [Online]. Available: https://sid.ir/paper/318356/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button