RECOMBINANT PRODUCTION OF MECASERMIN IN E. COLI EXPRESSION SYSTEM

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

JAFARI S.; BABAEIPOUR V.A.; RAHAIE M.; MOFID M.R.; HADDAD L.; NAMVARAN M.M.; FALLAH J.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: RESEARCH IN PHARMACEUTICAL SCIENCES (RPS) Year:2014 | Volume:9 | Issue:6 Page(s): 452-461

Download Full-Text

View:

344

Download:

Cites:

Information Journal Paper

Title

RECOMBINANT PRODUCTION OF MECASERMIN IN E. COLI EXPRESSION SYSTEM

Author(s)

Keywords

MECASERMIN

INSULIN-LIKE GROWTH FACTOR-1

OVER-EXPRESSION

INCLUSION BODY

PURIFICATION

WESTERN BLOTTING

Abstract

Human Insulin-like growth factor 1 (hIGF-1) consists of 70 amino acids in a single chain with three intermolecular disulfide bridges possessing valuable therapeutic effects. To date, numerous variants of specifically engineered hIGF-1 have been produced so as to improve hIGF-1 biological activity, stability and stronger binding to IGF-1 receptor. MECASERMIN is one of the modified variants with one amino acid substitution near the N-terminal (T4I) approved for the treatment of growth failure diabetes, wound healing, amyotrophic lateral sclerosis and severe primary IGF-1 deficiency. No scientific report for recombinant production of MECASERMIN in Escherichia coli (E. coli) expression system has been sofar reported. In the present study, we therefore investigated the overexpression of MECASERMIN in two different E. coli strains in order to obtain higher yield of recombinant protein. To achieve this goal, MECASERMIN DNA encoding sequence was designed based on polypeptide sequence, optimized according to E. coli codon preference, and cloned in pET15b. Recombinant vector, pET15-MECASERMIN, transferred into two E. coli strains rigami B (DE3) and BL21 (DE3) and induced for expression in a small scale. Results revealed the E. coli Origami B (DE3) expression system was a preferable host for MECASERMIN production due to its high expression level being around twice as much as BL21 (DE3). Large scale MECASERMIN production was performed in batch culture and produced recombinant protein specifically confirmed by WESTERN BLOTTING and mass spectroscopy.Since major part of recombinant MECASERMIN was expressed as INCLUSION BODY, isolation and refolding was accomplished through developed PURIFICATION procedure, and finally recombinant protein was successfully purified by gel filtration chromatography.

Cites

EXPRESSION OF RECOMBINANT HUMAN INSULIN-LIKE GROWTH FACTOR TYPE 1 (RHIGF-1) IN ESCHERICHIA COLI

References

No record.

Cite

APA: Copy

JAFARI, S., BABAEIPOUR, V.A., RAHAIE, M., MOFID, M.R., HADDAD, L., NAMVARAN, M.M., & FALLAH, J.. (2014). RECOMBINANT PRODUCTION OF MECASERMIN IN E. COLI EXPRESSION SYSTEM. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), 9(6), 452-461. SID. https://sid.ir/paper/318375/en

Vancouver: Copy

JAFARI S., BABAEIPOUR V.A., RAHAIE M., MOFID M.R., HADDAD L., NAMVARAN M.M., FALLAH J.. RECOMBINANT PRODUCTION OF MECASERMIN IN E. COLI EXPRESSION SYSTEM. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS)[Internet]. 2014;9(6):452-461. Available from: https://sid.ir/paper/318375/en

IEEE: Copy

S. JAFARI, V.A. BABAEIPOUR, M. RAHAIE, M.R. MOFID, L. HADDAD, M.M. NAMVARAN, and J. FALLAH, “RECOMBINANT PRODUCTION OF MECASERMIN IN E. COLI EXPRESSION SYSTEM,” RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), vol. 9, no. 6, pp. 452–461, 2014, [Online]. Available: https://sid.ir/paper/318375/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops