IMMUNIZATION OF MICE BY A MULTIMERIC L2-BASED LINEAR EPITOPE (17-36) FROM HPV TYPE 16/18 INDUCED CROSS REACTIVE NEUTRALIZING ANTIBODIES

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MOTAVALLI KHIAVI FARHAD; ARASHKIA ARASH; NASIMI MARYAM; MAHDAVI MEHDI; GOLKAR MAJID; ROOHVAND FARZIN; AZADMANESH KAYHAN

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Journal: RESEARCH IN PHARMACEUTICAL SCIENCES (RPS) Year:2017 | Volume:12 | Issue:4 Page(s): 265-273

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Title

IMMUNIZATION OF MICE BY A MULTIMERIC L2-BASED LINEAR EPITOPE (17-36) FROM HPV TYPE 16/18 INDUCED CROSS REACTIVE NEUTRALIZING ANTIBODIES

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Keywords

HUMAN PAPILLOMA VIRUS

CROSS NEUTRALIZATION

PSEUDOVIRUS AND L2 PEPTIDE

Abstract

Current licensed and commercially available prophylactic human papillomavirus (HPV) vaccines (Cervarix and quadrivalent/nine valents Gardasil) are based on major capsid protein L1 virus-like particles (VLPs) production which are expensive and type specific. Minor capsid L2-RG1 linear epitope (17-36) is a known candidate for induction of cross-neutralizing antibodies to develop low-cost pan-HPV vaccines. Herein, we report construction and expression of a three tandem repeats of L2-RG1 derived from HPV16 and 18 fused with the same head to tail pattern (HPV16: 17-36×3+HPV18: 17-36×3; hereafter termed dual-type fusion L2 peptide) in E. coli and provide the results of its immunogenicity in mice. SDS-PAGE and western blot analyses indicated proper expression of the peptide that could be further purified by Ni-NTA affinity chromatography via the located C-terminal 6xHis-tag. Mice immunized by formulation of the purified peptide and Freund adjuvant raised neutralizing antibodies which showed proper cross reactivity to HPV L2 (11-200) of types: 18, 16, 31 and 45 (which totally are responsible for 90% of cervical cancers) and efficiently neutralized HPV18/16 pseudovirusesin vitro. Our results imply the possibility of development of a simple, low-cost preventive HPV vaccine based on this dual-type fusion L2 peptide in bacterial expression system with broad spectrum.

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APA: Copy

MOTAVALLI KHIAVI, FARHAD, ARASHKIA, ARASH, NASIMI, MARYAM, MAHDAVI, MEHDI, GOLKAR, MAJID, ROOHVAND, FARZIN, & AZADMANESH, KAYHAN. (2017). IMMUNIZATION OF MICE BY A MULTIMERIC L2-BASED LINEAR EPITOPE (17-36) FROM HPV TYPE 16/18 INDUCED CROSS REACTIVE NEUTRALIZING ANTIBODIES. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), 12(4), 265-273. SID. https://sid.ir/paper/318464/en

Vancouver: Copy

MOTAVALLI KHIAVI FARHAD, ARASHKIA ARASH, NASIMI MARYAM, MAHDAVI MEHDI, GOLKAR MAJID, ROOHVAND FARZIN, AZADMANESH KAYHAN. IMMUNIZATION OF MICE BY A MULTIMERIC L2-BASED LINEAR EPITOPE (17-36) FROM HPV TYPE 16/18 INDUCED CROSS REACTIVE NEUTRALIZING ANTIBODIES. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS)[Internet]. 2017;12(4):265-273. Available from: https://sid.ir/paper/318464/en

IEEE: Copy

FARHAD MOTAVALLI KHIAVI, ARASH ARASHKIA, MARYAM NASIMI, MEHDI MAHDAVI, MAJID GOLKAR, FARZIN ROOHVAND, and KAYHAN AZADMANESH, “IMMUNIZATION OF MICE BY A MULTIMERIC L2-BASED LINEAR EPITOPE (17-36) FROM HPV TYPE 16/18 INDUCED CROSS REACTIVE NEUTRALIZING ANTIBODIES,” RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), vol. 12, no. 4, pp. 265–273, 2017, [Online]. Available: https://sid.ir/paper/318464/en

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