مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

271
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

132
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

The surface markers and survival rate of platelets during storage at 4° C: The influence of sodium octanoate

Pages

  105-116

Abstract

 Background: Storage of Platelet concentrates (PCs) at room temperature (20-24° C) limits its storage time to 5 days due to the destructive effects of Platelet storage lesion (PSL) and bacterial contamination. Although prolonged storage of Platelets (PLTs) at 4° C reduces the likelihood of bacterial contamination and PSL levels, it is accompanied by an increase in the clearance rate and changes in the surface markers of PLTs. The goal of this study was to evaluate the effects of Sodium Octanoate (SO) as a stabilizer on PLTs during storage at 4° C. Materials and Methods: In this experimental study, PCs were divided into three portions and stored for 5 days at 3 different conditions, including 20-24° C, 4° C temperature, and 4° C in presence of SO. PLTs enumeration was performed using an automated hematology analyzer. To measure the metabolic activity and survival rate of PLTs, the water-soluble tetrazolium salt (WST-1) assay was performed. The activity of lactate dehydrogenase enzyme (LDH) was measured by a biochemical analyzer. Additionally, the levels of PLT glycoprotein Ibα (GPIbα ) and CD62P (P-selectin) were measured on PLTs by flow cytometry technique. Results: PLTs count was higher in SO-treated (4° C) PLTs than two other studied samples. Additionally, the viability was higher in the SO-treated PLTs than that in other groups. LDH amount was lower in the SO-treated PLTs than that in other groups (P>0. 05). GPIbα expression was significantly higher in SO-treated PLTs than that other groups (P<0. 05). On the other hand, the expression of CD62P was lower at 4° C in PLTs in the presence of SO (P>0. 05). Conclusions: SO could modulate the effects of cold temperatures on PLTs. Furthermore, we found that the survival of Platelets was better maintained in the presence of SO at 4° C.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    Baghdadi, Vahid, YARI, FATEMEH, REZAEI, NEGAR, & RAFIEE, MOHAMMAD HESSAM. (2019). The surface markers and survival rate of platelets during storage at 4° C: The influence of sodium octanoate. IRANIAN JOURNAL OF PEDIATRIC HEMATOLOGY AND ONCOLOGY, 9(2), 105-116. SID. https://sid.ir/paper/320722/en

    Vancouver: Copy

    Baghdadi Vahid, YARI FATEMEH, REZAEI NEGAR, RAFIEE MOHAMMAD HESSAM. The surface markers and survival rate of platelets during storage at 4° C: The influence of sodium octanoate. IRANIAN JOURNAL OF PEDIATRIC HEMATOLOGY AND ONCOLOGY[Internet]. 2019;9(2):105-116. Available from: https://sid.ir/paper/320722/en

    IEEE: Copy

    Vahid Baghdadi, FATEMEH YARI, NEGAR REZAEI, and MOHAMMAD HESSAM RAFIEE, “The surface markers and survival rate of platelets during storage at 4° C: The influence of sodium octanoate,” IRANIAN JOURNAL OF PEDIATRIC HEMATOLOGY AND ONCOLOGY, vol. 9, no. 2, pp. 105–116, 2019, [Online]. Available: https://sid.ir/paper/320722/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button