The Effect of Platelet Lysate on Expansion and Differentiation Megakaryocyte Progenitor Cells from Cord Blood CD34+ enriched Cells

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Yaghoubi Yoda; HASSANZADEH ALI; Naimi Adel; Abdolahi Sepehr; YOUSEFI MEHDI; AGHEBATI MALEKI LEILI; ZAMANI MAJID

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Journal Paper

Paper Information

Journal: Iranian Journal of Pediatric Hematology Oncology Year:2021 | Volume:11 | Issue:3 Page(s): 172-182

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Title

The Effect of Platelet Lysate on Expansion and Differentiation Megakaryocyte Progenitor Cells from Cord Blood CD34+ enriched Cells

Author(s)

Keywords

Cord Blood Stem Cell Transplantation

Hematopoietic Stem Cells

Megakaryocyte Progenitor Cells

Platelet-Derived Growth Factor

Abstract

Background: Umbilical cord blood Hematopoietic Stem Cells (UCB-HSCs) are an attractive source for transplantation. The generation of megakaryocyte-committed cells could lead to shorten period of thrombocytopenia after HSCs transplantation. Platelet lysate (PL) unlike fetal bovine serum (FBS) can prevent immune problems as well as avert transmission of certain diseases to the recipient. In this study, the authors aimed to assess the effect of PL on UCB CD34+ cells expansion and megakaryocyte differentiation. Materials and Methods: In this experimental study, PL prepared and the subsequent isolation of UCB CD34+ cells were done by magnetic cell sorting. The isolated cells were cultivated in Iscove’ s Modified Dulbecco’ s medium (IMDM) supplemented with PL or FBS. Cell expansion was evaluated using Trypan blue. Furthermore, Flow cytometry using monoclonal antibodies (CD41-FITC and CD42b-PE) and the expression of specific genes including GATA1, GATA2, FLI1, NFE2, and RUNX1 via real-time PCR were performed to evaluate the megakaryocyte differentiation. Results: The results showed that PL insignificantly enhanced UCB CD34+ cell expansion (32. 83± 8. 47 fold in FBS and 41. 67± 10. 31 fold in PL containing media). Besides, flow cytometry results showed that expression of CD41 was increased markedly (37. 81± 4. 78 fold in FBS and 45. 78 ± 7. 37 in PL containing media, P-value <0. 05) but the elevation of CD42b (10. 53 ± 2. 13 and 13. 20 ± 2. 06 in FBS and PL containing media, respectively) was not significant (P-value = 0. 051). The results of real-time PCR demonstrated a notable increase in GATA binding protein 1 (1. 58, P-value <0. 01), GATA binding protein 2 (2. 45, P-value <0. 001), RUNX family transcription factor 1 (1. 60, P-value <0. 01), Fli-1 proto-oncogene (1. 87, P-value <0. 001) in PL supplemented media, however, the increase of Nuclear Factor-Erythroid 2 gene expression was not significant in PL supplemented media (Pvalue = 0. 11). Conclusion: PL improved UCB CD34+ cells expansion and megakaryocyte differentiation compared to FBS.

Cites

Efficient Production of Platelets from Umbilical Cord Blood Stem Cells Using A Collagen-Tragacanth Scaffold in A Multi-Chamber Bioreactor

References

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APA: Copy

Yaghoubi, Yoda, HASSANZADEH, ALI, Naimi, Adel, Abdolahi, Sepehr, YOUSEFI, MEHDI, AGHEBATI MALEKI, LEILI, & ZAMANI, MAJID. (2021). The Effect of Platelet Lysate on Expansion and Differentiation Megakaryocyte Progenitor Cells from Cord Blood CD34+ enriched Cells. IRANIAN JOURNAL OF PEDIATRIC HEMATOLOGY AND ONCOLOGY, 11(3), 172-182. SID. https://sid.ir/paper/979223/en

Vancouver: Copy

Yaghoubi Yoda, HASSANZADEH ALI, Naimi Adel, Abdolahi Sepehr, YOUSEFI MEHDI, AGHEBATI MALEKI LEILI, ZAMANI MAJID. The Effect of Platelet Lysate on Expansion and Differentiation Megakaryocyte Progenitor Cells from Cord Blood CD34+ enriched Cells. IRANIAN JOURNAL OF PEDIATRIC HEMATOLOGY AND ONCOLOGY[Internet]. 2021;11(3):172-182. Available from: https://sid.ir/paper/979223/en

IEEE: Copy

Yoda Yaghoubi, ALI HASSANZADEH, Adel Naimi, Sepehr Abdolahi, MEHDI YOUSEFI, LEILI AGHEBATI MALEKI, and MAJID ZAMANI, “The Effect of Platelet Lysate on Expansion and Differentiation Megakaryocyte Progenitor Cells from Cord Blood CD34+ enriched Cells,” IRANIAN JOURNAL OF PEDIATRIC HEMATOLOGY AND ONCOLOGY, vol. 11, no. 3, pp. 172–182, 2021, [Online]. Available: https://sid.ir/paper/979223/en

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