مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

140
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

81
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Expression of Collagen Type II and Osteocalcin Genes in Mesenchymal Stem Cells from Rats Treated with Lead acetate II

Pages

  35-40

Abstract

 Background: Lead is one of the sustainable metals with devastating effects on many tissues. This study, examined the adverse effect of lead poisoning on the gene expression of Collagen Type II and Osteocalcin by Mesenchymal Stem Cells (MSCs) cultured in chondrogenic and osteogenic media, respectively. Methods: We used 18 male Wistar rats, divided in 3 groups. In addition to libitum feed as the control, treatment I and treatment II groups were fed by distilled water, distilled water with a dose of 50 ppm Lead Acetate II and distilled water with a dose of 100 ppm Lead Acetate II, respectively, over a 2-month period. The MSCs of rat femur were isolated in DMEM medium. After the second passage, the media were replaced separately with chondrogenic and osteogenic media over another 21 days. Then, Collagen Type II and Osteocalcin genes expression were investigated by real time PCR. Results: Collagen Type II and Osteocalcin genes expression in treatments I and II groups showed meaningful decreases compared with that of the control group. Also, the concentration of Collagen Type II in treatment II group in chondrogenic medium was significantly reduced compared with Osteocalcin concentration in osteogenic medium. Conclusion: We found that poisoning with lead and its accumulation at doses of 50 and 100 ppm in femoral bone marrow of rats decreased the expression of the Collagen Type II and Osteocalcin genes in MSCs and in the chondrogenic and osteogenic media, respectively.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    RAFIEI, HOSEIN, & Ashrafizadeh, Milad. (2018). Expression of Collagen Type II and Osteocalcin Genes in Mesenchymal Stem Cells from Rats Treated with Lead acetate II. IRANIAN JOURNAL OF TOXICOLOGY, 12(5), 35-40. SID. https://sid.ir/paper/324515/en

    Vancouver: Copy

    RAFIEI HOSEIN, Ashrafizadeh Milad. Expression of Collagen Type II and Osteocalcin Genes in Mesenchymal Stem Cells from Rats Treated with Lead acetate II. IRANIAN JOURNAL OF TOXICOLOGY[Internet]. 2018;12(5):35-40. Available from: https://sid.ir/paper/324515/en

    IEEE: Copy

    HOSEIN RAFIEI, and Milad Ashrafizadeh, “Expression of Collagen Type II and Osteocalcin Genes in Mesenchymal Stem Cells from Rats Treated with Lead acetate II,” IRANIAN JOURNAL OF TOXICOLOGY, vol. 12, no. 5, pp. 35–40, 2018, [Online]. Available: https://sid.ir/paper/324515/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button