COMBINED SODIUM HYPOCHLORITE AND 940 NM DIODE LASER TREATMENT AGAINST MATURE E. FAECALIS BIOFILMS IN-VITRO

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CASTELO BAZ PABLO; MARTIN BIEDMA BENJAMIN; RUIZ PINON MANUEL; RIVAS MUNDINA BERTA; BAHILLO JOSE; PEREZ ESTEVEZ ANTONIO; GUDE FRANCISCO; MOOR ROELAND DE; VARELA PATINO PURIFICACION

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Journal: JOURNAL OF LASERS IN MEDICAL SCIENCES Year:2012 | Volume:3 | Issue:3 Page(s): 116-121

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Title

COMBINED SODIUM HYPOCHLORITE AND 940 NM DIODE LASER TREATMENT AGAINST MATURE E. FAECALIS BIOFILMS IN-VITRO

Author(s)

Keywords

E. FAECALIS

DIODE LASER

BIOFILM

DISINFECTION

Abstract

Background: Laser irradiation has been introduced in endodontic treatment due to its bactericidal effect. The aim of this study is to evaluate the bactericidal efficacy of a 940 nm DIODE LASER alone or in combination with 5% sodium hypochlorite (NaOCl) against mature BIOFILMs of E. FAECALIS.Methods: Sixty-eight (60 for the three groups, 4 for SEM and 4 as negative controls) single-rooted human central incisors were prepared and contaminated with E. FAECALIS. After two weeks of incubation, specimens were randomly divided in three groups; group 1 (n =20), the teeth were irradiated with a 940 nm DIODE LASER; group 2 (n=20), specimens were rinsed with 5% NaOCl; group 3 (n=20), the teeth were rinsed with 5% NaOCl and then were irradiated with 940 nm DIODE LASER. Four teeth were used to observe the BIOFILMs by SEM. Intracanal bacteria sampling was done, and the samples were plated to determinate the CFU count.Results: At 24 hours and 7 days, group 3 showed a significant difference (P=0.02; P=0.00) in DISINFECTION if compared to group 1 but did not show this difference if compared to group 2 (P=1, P=0.66), although group 3 obtaining a more extensive DISINFECTION. Groups 1 and 2 did not show difference after 24 hours (P=0.09) but showed a significant difference 7 days afterwards (P=0.04).Conclusion: The combination of sodium hypochlorite and DIODE LASER light (940 nm) has a synergistic effect, intensifying the bactericidal action.

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APA: Copy

CASTELO BAZ, PABLO, MARTIN BIEDMA, BENJAMIN, RUIZ PINON, MANUEL, RIVAS MUNDINA, BERTA, BAHILLO, JOSE, PEREZ ESTEVEZ, ANTONIO, GUDE, FRANCISCO, MOOR, ROELAND DE, & VARELA PATINO, PURIFICACION. (2012). COMBINED SODIUM HYPOCHLORITE AND 940 NM DIODE LASER TREATMENT AGAINST MATURE E. FAECALIS BIOFILMS IN-VITRO. JOURNAL OF LASERS IN MEDICAL SCIENCES, 3(3), 116-121. SID. https://sid.ir/paper/325811/en

Vancouver: Copy

CASTELO BAZ PABLO, MARTIN BIEDMA BENJAMIN, RUIZ PINON MANUEL, RIVAS MUNDINA BERTA, BAHILLO JOSE, PEREZ ESTEVEZ ANTONIO, GUDE FRANCISCO, MOOR ROELAND DE, VARELA PATINO PURIFICACION. COMBINED SODIUM HYPOCHLORITE AND 940 NM DIODE LASER TREATMENT AGAINST MATURE E. FAECALIS BIOFILMS IN-VITRO. JOURNAL OF LASERS IN MEDICAL SCIENCES[Internet]. 2012;3(3):116-121. Available from: https://sid.ir/paper/325811/en

IEEE: Copy

PABLO CASTELO BAZ, BENJAMIN MARTIN BIEDMA, MANUEL RUIZ PINON, BERTA RIVAS MUNDINA, JOSE BAHILLO, ANTONIO PEREZ ESTEVEZ, FRANCISCO GUDE, ROELAND DE MOOR, and PURIFICACION VARELA PATINO, “COMBINED SODIUM HYPOCHLORITE AND 940 NM DIODE LASER TREATMENT AGAINST MATURE E. FAECALIS BIOFILMS IN-VITRO,” JOURNAL OF LASERS IN MEDICAL SCIENCES, vol. 3, no. 3, pp. 116–121, 2012, [Online]. Available: https://sid.ir/paper/325811/en

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