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Information Journal Paper

Title

Silymarin inhibits Toll-like receptor 8 gene expression and apoptosis in Ramos cancer cell line

Pages

  161-169

Abstract

 Objective: Silymarin is a herbal extract containing flavonolignans, and it has inhibitory effects against the growth of different cancer cell lines by inducing Apoptosis. Toll-like receptors are suggested as a novel and attractive target to treat cancer. The current study aimed at examining the mechanism of Silymarin-induced Apoptosis in Ramos cells and investigating its effects on TLR8 expression. Materials and Methods: The half maximal inhibitory concentration (IC50) of Silymarin in Ramos cells was determined via MTT viability test while the type of cell death was tested by annexin V/propidium iodide (PI) double staining method. The activity of caspase-3 and expression of TLR8 were measured in a time-dependent manner (in IC50) by colorimetric assay and real-time polymerase chain reaction (RT-PCR), respectively. Results: The results of MTT showed that IC50 of Silymarin in Ramos cells was 100 μ g/ml after 48 hr treatment (p<0. 01). Flow cytometry by annexin V/PI, showed that Silymarin induced early/late Apoptosis in this cell line (p<0. 05 to p<0. 01). In addition, the caspase-3 colorimetric method showed that caspase-3 increased in the Ramos cell line after treatment (p<0. 01). This treatment led to a reduction in TLR8 mRNA expression in a time-dependent manner (p<0. 01). Conclusion: The results indicated a new mechanism in the anticancer activity of Toll-like receptor (TLR) signaling after Silymarin treatment in Ramos cancer cell line. This plant could be used to develop anticancer agents inhibiting TLRs.

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    APA: Copy

    FAEZIZADEH, ZOHREH. (2020). Silymarin inhibits Toll-like receptor 8 gene expression and apoptosis in Ramos cancer cell line. AVICENNA JOURNAL OF PHYTOMEDICINE (AJP), 10(2), 161-169. SID. https://sid.ir/paper/326485/en

    Vancouver: Copy

    FAEZIZADEH ZOHREH. Silymarin inhibits Toll-like receptor 8 gene expression and apoptosis in Ramos cancer cell line. AVICENNA JOURNAL OF PHYTOMEDICINE (AJP)[Internet]. 2020;10(2):161-169. Available from: https://sid.ir/paper/326485/en

    IEEE: Copy

    ZOHREH FAEZIZADEH, “Silymarin inhibits Toll-like receptor 8 gene expression and apoptosis in Ramos cancer cell line,” AVICENNA JOURNAL OF PHYTOMEDICINE (AJP), vol. 10, no. 2, pp. 161–169, 2020, [Online]. Available: https://sid.ir/paper/326485/en

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