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Information Journal Paper

Title

Short-Communication: The mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death

Pages

  491-498

Abstract

 Objective: Oxidative stress is associated with the pathogenesis of brain ischemia and other neurodegenerative disorders. Previous researches have shown the antioxidant activity of Viola odorata L. In this project, we studied neuro-protective and reactive oxygen species (ROS) scavenging activities of methanol (MeOH) extract and other fractions isolated from V. odorata in PC12 cell line in serum/glucose deprivation (SGD) condition. Materials and Methods: The PC12 neuronal cells were pretreated for 6 hr with MeOH extract and fractions of V. odorata (1 to 25 μ g/ml) followed by 24 hr incubation under SGD condition. Cell viability was measured by Alamar Blue ® assay. The level of ROS was calculated using DCFH-DA. Also, Bax/Bcl-2 protein ratio was analyzed by western blot assay. Results: SGD condition significantly decreased cells viability (p<0. 001). Pretreatment with EtOAc (12. 5 and 25 µ g/ml), BuOH (12, 25, 50 µ g/ml) and CH2Cl2 (1. 5 µ g/ml) fractions of V. odorata reduced SGD-induced cytotoxicity. MeOH extract could not increase the viability significantly. All four semi polar fractions (EtOAc, BuOH, CH2Cl2 and MeOH) decreased SGD-induced ROS production and changed Bax/Bcl-2 ratio. Conclusion: V. odorata showed promising effects against SGD condition; further mechanistic and clinical studies are warranted before application of V. odorata as a neuro-protective agent.

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    APA: Copy

    TAYARANI NAJARAN, ZAHRA, YAZDIAN ROBATI, REZVAN, AMINI, ELAHEH, Salek, Farzaneh, Arasteh, Fatemeh, & EMAMI, SEYED AHMAD. (2019). Short-Communication: The mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death. AVICENNA JOURNAL OF PHYTOMEDICINE (AJP), 9(6), 491-498. SID. https://sid.ir/paper/326538/en

    Vancouver: Copy

    TAYARANI NAJARAN ZAHRA, YAZDIAN ROBATI REZVAN, AMINI ELAHEH, Salek Farzaneh, Arasteh Fatemeh, EMAMI SEYED AHMAD. Short-Communication: The mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death. AVICENNA JOURNAL OF PHYTOMEDICINE (AJP)[Internet]. 2019;9(6):491-498. Available from: https://sid.ir/paper/326538/en

    IEEE: Copy

    ZAHRA TAYARANI NAJARAN, REZVAN YAZDIAN ROBATI, ELAHEH AMINI, Farzaneh Salek, Fatemeh Arasteh, and SEYED AHMAD EMAMI, “Short-Communication: The mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death,” AVICENNA JOURNAL OF PHYTOMEDICINE (AJP), vol. 9, no. 6, pp. 491–498, 2019, [Online]. Available: https://sid.ir/paper/326538/en

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